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Literature summary for 2.3.3.17 extracted from

  • Textor, S.; de Kraker, J.W.; Hause, B.; Gershenzon, J.; Tokuhisa, J.G.
    MAM3 catalyzes the formation of all aliphatic glucosinolate chain lengths in Arabidopsis (2007), Plant Physiol., 144, 60-71.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol isoform MAM3 has no requirement for dithiothreitol Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Arabidopsis thaliana
EDTA complete inhibition at 0.04 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.081
-
9-methylthio-2-oxononanoate at pH 8.0 and 32°C Arabidopsis thaliana
0.253
-
8-methylthio-2-oxooctanoate at pH 8.0 and 32°C Arabidopsis thaliana
0.463
-
6-methylthio-2-oxohexanoate at pH 8.0 and 32°C Arabidopsis thaliana
0.476
-
5-methylthio-2-oxopentanoate at pH 8.0 and 32°C Arabidopsis thaliana
0.932
-
4-methylthio-2-oxobutanoate at pH 8.0 and 32°C Arabidopsis thaliana
1
-
2-oxoisovalerate at pH 8.0 and 32°C Arabidopsis thaliana
2.3
-
acetyl-CoA at pH 8.0 and 32°C Arabidopsis thaliana
8.6
-
pyruvate at pH 8.0 and 32°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ highest activity with Mn2+ Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
x * 53000, SDS-PAGE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FN52 ecotype Columbia
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoisovalerate + acetyl-CoA
-
Arabidopsis thaliana isopropylmalate + CoA
-
?
4-methyl-2-oxopentanoate + acetyl-CoA
-
Arabidopsis thaliana ?
-
?
4-methylthio-2-oxobutanoate + acetyl-CoA + H2O
-
Arabidopsis thaliana 2-(2'-methylthioethyl)-malate + CoA
-
?
5-methyl-2-oxohexanoate + acetyl-CoA
-
Arabidopsis thaliana ?
-
?
5-methylthio-2-oxopentanoate + acetyl-CoA
-
Arabidopsis thaliana ?
-
?
6-methylthio-2-oxohexanoate + acetyl-CoA + H2O
-
Arabidopsis thaliana 2-(4-methylthiobutyl)malate + CoA
-
?
8-methylthio-2-oxooctanoate + acetyl-CoA + H2O
-
Arabidopsis thaliana 2-(6-methylthiohexyl)malate + CoA
-
?
9-methylthio-2-oxononanoate + acetyl-CoA
-
Arabidopsis thaliana ?
-
?
additional information 2-oxo-dodecanoate is not a substrate for isoform MAM3 Arabidopsis thaliana ?
-
?
pyruvate + acetyl-CoA
-
Arabidopsis thaliana citramalate + CoA
-
?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
MAM3 isoform, formerly known as MAM-L Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
-
Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 39 70% activity at 25°C and 39°C Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
9-methylthio-2-oxononanoate at pH 8.0 and 32°C Arabidopsis thaliana
0.17
-
pyruvate at pH 8.0 and 32°C Arabidopsis thaliana
0.18
-
2-oxoisovalerate at pH 8.0 and 32°C Arabidopsis thaliana
0.3
-
8-methylthio-2-oxooctanoate at pH 8.0 and 32°C Arabidopsis thaliana
1.3
-
4-methylthio-2-oxobutanoate at pH 8.0 and 32°C Arabidopsis thaliana
1.3
-
5-methylthio-2-oxopentanoate at pH 8.0 and 32°C Arabidopsis thaliana
2.5
-
6-methylthio-2-oxohexanoate at pH 8.0 and 32°C Arabidopsis thaliana
3
-
acetyl-CoA at pH 8.0 and 32°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
6.3 9.2 50% activity at pH 6.3 and 9.0 Arabidopsis thaliana

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.01
-
at pH 8.0 and 32°C Arabidopsis thaliana EDTA
18
-
at pH 8.0 and 32°C Arabidopsis thaliana ATP

General Information

General Information Comment Organism
metabolism isoform MAM3 catalyzes the formation of all glucosinolate chain lengths in vivo Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.023
-
pyruvate at pH 8.0 and 32°C Arabidopsis thaliana
0.2
-
2-oxoisovalerate at pH 8.0 and 32°C Arabidopsis thaliana
0.37
-
9-methylthio-2-oxononanoate at pH 8.0 and 32°C Arabidopsis thaliana
1.28
-
8-methylthio-2-oxooctanoate at pH 8.0 and 32°C Arabidopsis thaliana
1.3
-
acetyl-CoA at pH 8.0 and 32°C Arabidopsis thaliana
1.38
-
4-methylthio-2-oxobutanoate at pH 8.0 and 32°C Arabidopsis thaliana
2.73
-
5-methylthio-2-oxopentanoate at pH 8.0 and 32°C Arabidopsis thaliana
5.4
-
6-methylthio-2-oxohexanoate at pH 8.0 and 32°C Arabidopsis thaliana