Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | isoform MAM3 has no requirement for dithiothreitol | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana | |
EDTA | complete inhibition at 0.04 mM | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.081 | - |
9-methylthio-2-oxononanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
0.253 | - |
8-methylthio-2-oxooctanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
0.463 | - |
6-methylthio-2-oxohexanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
0.476 | - |
5-methylthio-2-oxopentanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
0.932 | - |
4-methylthio-2-oxobutanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
1 | - |
2-oxoisovalerate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3 | - |
acetyl-CoA | at pH 8.0 and 32°C | Arabidopsis thaliana | |
8.6 | - |
pyruvate | at pH 8.0 and 32°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | highest activity with Mn2+ | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, SDS-PAGE | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9FN52 | ecotype Columbia | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoisovalerate + acetyl-CoA | - |
Arabidopsis thaliana | isopropylmalate + CoA | - |
? | |
4-methyl-2-oxopentanoate + acetyl-CoA | - |
Arabidopsis thaliana | ? | - |
? | |
4-methylthio-2-oxobutanoate + acetyl-CoA + H2O | - |
Arabidopsis thaliana | 2-(2'-methylthioethyl)-malate + CoA | - |
? | |
5-methyl-2-oxohexanoate + acetyl-CoA | - |
Arabidopsis thaliana | ? | - |
? | |
5-methylthio-2-oxopentanoate + acetyl-CoA | - |
Arabidopsis thaliana | ? | - |
? | |
6-methylthio-2-oxohexanoate + acetyl-CoA + H2O | - |
Arabidopsis thaliana | 2-(4-methylthiobutyl)malate + CoA | - |
? | |
8-methylthio-2-oxooctanoate + acetyl-CoA + H2O | - |
Arabidopsis thaliana | 2-(6-methylthiohexyl)malate + CoA | - |
? | |
9-methylthio-2-oxononanoate + acetyl-CoA | - |
Arabidopsis thaliana | ? | - |
? | |
additional information | 2-oxo-dodecanoate is not a substrate for isoform MAM3 | Arabidopsis thaliana | ? | - |
? | |
pyruvate + acetyl-CoA | - |
Arabidopsis thaliana | citramalate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
MAM3 | isoform, formerly known as MAM-L | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 39 | 70% activity at 25°C and 39°C | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
9-methylthio-2-oxononanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
0.17 | - |
pyruvate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
0.18 | - |
2-oxoisovalerate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
0.3 | - |
8-methylthio-2-oxooctanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
1.3 | - |
4-methylthio-2-oxobutanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
1.3 | - |
5-methylthio-2-oxopentanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.5 | - |
6-methylthio-2-oxohexanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
3 | - |
acetyl-CoA | at pH 8.0 and 32°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 9.2 | 50% activity at pH 6.3 and 9.0 | Arabidopsis thaliana |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.01 | - |
at pH 8.0 and 32°C | Arabidopsis thaliana | EDTA | |
18 | - |
at pH 8.0 and 32°C | Arabidopsis thaliana | ATP |
General Information | Comment | Organism |
---|---|---|
metabolism | isoform MAM3 catalyzes the formation of all glucosinolate chain lengths in vivo | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
pyruvate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
0.2 | - |
2-oxoisovalerate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
0.37 | - |
9-methylthio-2-oxononanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
1.28 | - |
8-methylthio-2-oxooctanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
1.3 | - |
acetyl-CoA | at pH 8.0 and 32°C | Arabidopsis thaliana | |
1.38 | - |
4-methylthio-2-oxobutanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.73 | - |
5-methylthio-2-oxopentanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
5.4 | - |
6-methylthio-2-oxohexanoate | at pH 8.0 and 32°C | Arabidopsis thaliana |