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Literature summary for 2.3.3.16 extracted from
- Expression, reactivation, and purification of enzymes from Haloferax volcanii in Escherichia coli. (1999), Biotechnol. Bioeng., 64, 38-45.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli using the cytoplasmic expression vectors, pET3a and pET3d. The inactive enzyme is reactivated following overnight incubation in 2 M KCl | Haloferax volcanii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | at 0.2 M KCl, approximately 60% of its maximal activity | Haloferax volcanii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.026 | - |
oxaloacetate | 30°C, pH 8.0, native enzyme | Haloferax volcanii | |
| 0.027 | - |
oxaloacetate | 30°C, pH 8.0, recombinant enzyme | Haloferax volcanii | |
| 123 | - |
acetyl-CoA | 30°C, pH 8.0, recombinant enzyme | Haloferax volcanii | |
| 125 | - |
acetyl-CoA | 30°C, pH 8.0, native enzyme | Haloferax volcanii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
x * 42000, SDS-PAGE | Haloferax volcanii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haloferax volcanii | D4GS06 | - |
- |
| Haloferax volcanii DSM 3757 | D4GS06 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recovered using dye-affinity chromatography in the presence of salt | Haloferax volcanii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 40 | - |
37°C, pH 8.0 | Haloferax volcanii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| room temperature, at 0.2 M KCl, the enzyme is stable at for at least 48 h | Haloferax volcanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + H2O + oxaloacetate | - |
Haloferax volcanii | citrate + CoA | - |
? | |
| acetyl-CoA + H2O + oxaloacetate | - |
Haloferax volcanii DSM 3757 | citrate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, SDS-PAGE | Haloferax volcanii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Haloferax volcanii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Haloferax volcanii |
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