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Literature summary for 2.3.3.16 extracted from

  • Kurz, L.C.; Constantine, C.Z.; Jiang, H.; Kappock, T.J.
    The partial substrate dethiaacetyl-coenzyme A mimics all critical carbon acid reactions in the condensation half-reaction catalyzed by Thermoplasma acidophilum citrate synthase (2009), Biochemistry, 48, 7878-7891.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.16

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermoplasma acidophilum

Protein Variants

Protein Variants Comment Organism
D12N reference for pI-value analysis Thermoplasma acidophilum
D317G D317 removes the acetyl-CoA methyl proton during catalysis Thermoplasma acidophilum
D317N D317 removes the acetyl-CoA methyl proton during catalysis Thermoplasma acidophilum
H187Q active site Thermoplasma acidophilum
H222Q active site Thermoplasma acidophilum
H262Q active site Thermoplasma acidophilum
W245F/W115F/W17F containing a single W (W348) Thermoplasma acidophilum
W348Y primary emitter in fluorescence analysis Thermoplasma acidophilum

Inhibitors

Inhibitors Comment Organism Structure
dethiaacetyl-CoA partial substrate Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum P21553
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + acetyl-CoA + H2O
-
 Thermoplasma acidophilum citrate + CoA
-
 ?

Synonyms

Synonyms Comment Organism
citrate synthase
-
 Thermoplasma acidophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0001
-
 acetyl-CoA D317N mutant protein, value is very uncertain Thermoplasma acidophilum
0.0001
-
 oxaloacetate D317N mutant protein, value is very uncertain Thermoplasma acidophilum
0.0015
-
 acetyl-CoA D317G mutant protein Thermoplasma acidophilum
0.0015
-
 oxaloacetate D317G mutant protein Thermoplasma acidophilum
9.2
-
 acetyl-CoA wild-type protein Thermoplasma acidophilum
9.2
-
 oxaloacetate wild-type protein Thermoplasma acidophilum

pI Value

Organism Comment pI Value Maximum pI Value
Thermoplasma acidophilum W245F/W115F/W17F mutant protein, isoelectric focusing
-
 8.6
Thermoplasma acidophilum D317G mutant protein, isoelectric focusing
-
 8.8
Thermoplasma acidophilum D317N mutant protein, isoelectric focusing
-
 8.8
Thermoplasma acidophilum wild-type protein, isoelectric focusing
-
 8.9
Thermoplasma acidophilum D12N mutant protein, isoelectric focusing
-
 9.1