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Literature summary for 2.3.3.16 extracted from

  • Kurz, L.C.; Fite, B.; Jean, J.; Park, J.; Erpelding, T.; Callis, P.
    Photophysics of tryptophan fluorescence: link with the catalytic strategy of the citrate synthase from Thermoplasma acidophilum (2005), Biochemistry, 44, 1394-1413.
    View publication on PubMed
Search for more literature for 2.3.3.16

Crystallization (Commentary)

Crystallization (Comment) Organism
unliganded form and the enzyme in complex with oxaloacetate. Oxaloacetate is most likely the quencher of tryptophan fluorescence with the oxaloacetate carbonyl as the electron acceptor Thermoplasma acidophilum

Protein Variants

Protein Variants Comment Organism
H187Q analysis of tryptophan fluorescence Thermoplasma acidophilum
R344K analysis of tryptophan fluorescence Thermoplasma acidophilum
W245F/W115F/W17F analysis of tryptophan flourescence Thermoplasma acidophilum
W348Y analysis of tryptophan fluorescence, residue responsible for quenching effect Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 citrate mutant H187Q, pH 8.0, 20°C Thermoplasma acidophilum
0.0005
-
 citrate mutant W348Y, pH 8.0, 20°C Thermoplasma acidophilum
0.00067
-
 acetyl-CoA wild-type, pH 8.0, 20°C Thermoplasma acidophilum
0.0007
-
 citrate mutant W245F/W115F/W17F, pH 8.0, 20°C Thermoplasma acidophilum
0.00085
-
 acetyl-CoA mutant W348Y, pH 8.0, 20°C Thermoplasma acidophilum
0.001
-
 citrate wild-type, pH 8.0, 20°C Thermoplasma acidophilum
0.003
-
 oxaloacetate mutant W245F/W115F/W17F, pH 8.0, 20°C Thermoplasma acidophilum
0.003
-
 oxaloacetate mutant W348Y, pH 8.0, 20°C Thermoplasma acidophilum
0.003
-
 oxaloacetate wild-type, pH 8.0, 20°C Thermoplasma acidophilum
0.0036
-
 acetyl-CoA mutant W245F/W115F/W17F, pH 8.0, 20°C Thermoplasma acidophilum
0.01
-
 acetyl-CoA mutant H187Q, pH 8.0, 20°C Thermoplasma acidophilum
0.017
-
 acetyl-CoA mutant R344K, pH 8.0, 20°C Thermoplasma acidophilum
0.054
-
 oxaloacetate mutant H187Q, pH 8.0, 20°C Thermoplasma acidophilum
0.37
-
 oxaloacetate mutant R344K, pH 8.0, 20°C Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate
-
 Thermoplasma acidophilum citrate + CoA
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03 0.55 acetyl-CoA mutant W348Y, pH 8.0, 20°C Thermoplasma acidophilum
0.5
-
 citrate mutant H187Q, pH 8.0, 20°C Thermoplasma acidophilum
6.73
-
 acetyl-CoA mutant W348Y, pH 8.0, 20°C Thermoplasma acidophilum
7.75
-
 citrate mutant W348Y, pH 8.0, 20°C Thermoplasma acidophilum
9.8
-
 acetyl-CoA wild-type, pH 8.0, 20°C Thermoplasma acidophilum
11
-
 acetyl-CoA mutant H187Q, pH 8.0, 20°C Thermoplasma acidophilum
11.4
-
 citrate wild-type, pH 8.0, 20°C Thermoplasma acidophilum
12.66
-
 citrate mutant H187Q, pH 8.0, 20°C Thermoplasma acidophilum
14.5
-
 acetyl-CoA mutant R344K, pH 8.0, 20°C Thermoplasma acidophilum
15.4
-
 acetyl-CoA mutant W245F/W115F/W17F, pH 8.0, 20°C Thermoplasma acidophilum
17.3
-
 citrate mutant W245F/W115F/W17F, pH 8.0, 20°C Thermoplasma acidophilum

pI Value

Organism Comment pI Value Maximum pI Value
Thermoplasma acidophilum mutant W245F/W115F/W17F, isoelectric focusing
-
 8.6
Thermoplasma acidophilum isoelectric focusing, wild-type
-
 8.8
Thermoplasma acidophilum mutant H187Q, isoelectric focusing
-
 8.8
Thermoplasma acidophilum mutant H222Q, isoelectric focusing
-
 8.8
Thermoplasma acidophilum mutant W348Y, isoelectric focusing
-
 8.8