BRENDA - Enzyme Database show
show all sequences of 2.3.3.16

Characterization of citrate synthase from Geobacter sulfurreducens and evidence for a family of citrate synthases similar to those of eukaryotes throughout the Geobacteraceae

Bond, D.R.; Mester, T.; Nesbo, C.L.; Izquierdo-Lopez, A.V.; Collart, F.L.; Lovley, D.R.; Appl. Environ. Microbiol. 71, 3858-3865 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Geobacter sulfurreducens
Inhibitors
Inhibitors
Commentary
Organism
Structure
ATP
-
Geobacter sulfurreducens
additional information
no inhibition by NADH or 2-oxaloacetate
Geobacter sulfurreducens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0043
-
oxaloacetate
30°C, pH 8, native enzyme
Geobacter sulfurreducens
0.0081
-
oxaloacetate
30°C, pH 8, recombinant enzyme
Geobacter sulfurreducens
0.0123
-
acetyl-CoA
30°C, pH 8, recombinant enzyme
Geobacter sulfurreducens
0.0141
-
acetyl-CoA
30°C, pH 8, native enzyme
Geobacter sulfurreducens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Geobacter sulfurreducens
Q6JGI2
-
-
Purification (Commentary)
Commentary
Organism
-
Geobacter sulfurreducens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + oxaloacetate + H2O
-
657610
Geobacter sulfurreducens
citrate + CoA
-
-
-
?
additional information
the enzyme does not have any methylcitrate synthase activity
657610
Geobacter sulfurreducens
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
-
Geobacter sulfurreducens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8.3
-
acetyl-CoA
30°C, pH 8, native enzyme
Geobacter sulfurreducens
8.3
-
oxaloacetate
30°C, pH 8, native enzyme
Geobacter sulfurreducens
8.8
-
acetyl-CoA
30°C, pH 8, recombinant enzyme
Geobacter sulfurreducens
8.8
-
oxaloacetate
30°C, pH 8, recombinant enzyme
Geobacter sulfurreducens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Geobacter sulfurreducens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10
about 30% of maximal activity at pH 6 and at pH 10
Geobacter sulfurreducens
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.9
-
ATP
30°C, pH 8, versus acetyl-CoA
Geobacter sulfurreducens
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Geobacter sulfurreducens
calculation from nucleotide sequence
-
6.46
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Geobacter sulfurreducens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ATP
-
Geobacter sulfurreducens
additional information
no inhibition by NADH or 2-oxaloacetate
Geobacter sulfurreducens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.9
-
ATP
30°C, pH 8, versus acetyl-CoA
Geobacter sulfurreducens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0043
-
oxaloacetate
30°C, pH 8, native enzyme
Geobacter sulfurreducens
0.0081
-
oxaloacetate
30°C, pH 8, recombinant enzyme
Geobacter sulfurreducens
0.0123
-
acetyl-CoA
30°C, pH 8, recombinant enzyme
Geobacter sulfurreducens
0.0141
-
acetyl-CoA
30°C, pH 8, native enzyme
Geobacter sulfurreducens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Geobacter sulfurreducens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + oxaloacetate + H2O
-
657610
Geobacter sulfurreducens
citrate + CoA
-
-
-
?
additional information
the enzyme does not have any methylcitrate synthase activity
657610
Geobacter sulfurreducens
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
-
Geobacter sulfurreducens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8.3
-
acetyl-CoA
30°C, pH 8, native enzyme
Geobacter sulfurreducens
8.3
-
oxaloacetate
30°C, pH 8, native enzyme
Geobacter sulfurreducens
8.8
-
acetyl-CoA
30°C, pH 8, recombinant enzyme
Geobacter sulfurreducens
8.8
-
oxaloacetate
30°C, pH 8, recombinant enzyme
Geobacter sulfurreducens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Geobacter sulfurreducens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10
about 30% of maximal activity at pH 6 and at pH 10
Geobacter sulfurreducens
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Geobacter sulfurreducens
calculation from nucleotide sequence
-
6.46
Other publictions for EC 2.3.3.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747131
Reddick
First biochemical characteriz ...
Bacillus subtilis 168, Bacillus subtilis
Biochemistry
56
5698-5711
2017
-
-
1
-
-
-
-
-
-
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4
-
5
-
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1
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8
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1
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4
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1
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8
-
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-
-
-
-
1
1
-
-
-
735413
Xu
-
Ethanol content in plants of B ...
Brassica napus
Acta Physiol. Plant.
38
1-9
2016
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
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1
-
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1
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1
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1
-
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1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
735400
Murphy
An active site-tail interactio ...
Thermoplasma acidophilum
Acta Crystallogr. Sect. F
71
1292-1299
2015
-
-
1
1
1
-
1
2
-
-
-
1
-
1
-
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1
-
-
-
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1
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1
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1
1
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1
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2
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1
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1
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1
-
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-
-
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-
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-
735799
Ge
Identification and characteriz ...
Streptomyces diastaticus, Streptomyces diastaticus SM33
Biotechnol. Appl. Biochem.
62
300-308
2015
1
-
1
-
-
-
11
2
-
3
3
2
-
2
-
-
1
-
-
-
-
-
2
1
1
-
1
2
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
11
-
2
-
3
3
2
-
-
-
1
-
-
-
-
2
1
1
-
1
2
1
-
-
-
-
-
-
-
2
2
736278
Roy
-
Insight into sequence, structu ...
Homo sapiens
Int. J. Pharm. Bio Sci.
6
B1309-B1321
2015
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
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-
-
-
-
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1
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-
-
-
-
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1
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1
-
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-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
737214
Ferraris
Structures of citrate synthase ...
Mycobacterium tuberculosis
Proteins
83
389-394
2015
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
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-
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1
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1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736463
Kobylarz
SbnG, a citrate synthase in St ...
Staphylococcus aureus
J. Biol. Chem.
289
33797-33807
2014
-
-
1
1
6
-
3
-
-
-
2
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
6
-
-
3
-
-
-
-
2
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736555
Rozycki
Citrate synthase proteins in e ...
Pyrobaculum aerophilum, Pyrococcus furiosus, Sus scrofa
J. Chem. Phys.
141
235102
2014
-
-
-
-
-
-
-
-
1
-
-
3
-
3
-
-
-
-
-
-
-
-
3
3
-
-
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-
-
-
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-
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-
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1
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3
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-
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-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736830
Sheldon
TCA cycle activity in Staphylo ...
Staphylococcus aureus, Staphylococcus aureus Newman
Mol. Microbiol.
92
824-839
2014
-
-
-
-
-
-
-
-
-
-
-
2
-
8
-
-
-
-
-
-
-
-
2
-
-
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-
-
-
-
-
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-
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-
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-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
3
3
1
-
-
736845
Schmidtmann
Redox regulation of Arabidopsi ...
Arabidopsis thaliana
Mol. Plant
7
156-169
2014
1
-
1
-
-
-
1
2
2
-
1
1
-
2
-
-
1
-
-
-
-
-
1
1
1
1
-
-
1
1
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
2
2
-
1
1
-
-
-
1
-
-
-
-
1
1
1
1
-
-
1
1
-
-
-
-
-
-
2
2
737011
Han
-
Isolation and preliminary func ...
Malus baccata var. xiaojinensis
Plant Mol. Biol. Rep.
33
133-142
2014
-
-
1
-
-
-
-
-
2
-
1
1
-
1
-
-
-
-
-
3
-
-
1
1
-
-
-
-
-
-
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1
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-
1
-
-
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-
-
-
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2
-
1
1
-
-
-
-
-
3
-
-
1
1
-
-
-
-
-
-
-
1
1
1
1
1
-
-
735698
Duckworth
Enzyme-substrate complexes of ...
Escherichia coli
Biochim. Biophys. Acta
1834
2546-2553
2013
-
-
-
-
3
-
1
5
-
1
1
1
-
1
-
-
-
-
-
-
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-
1
1
-
-
-
3
-
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-
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-
-
-
-
3
-
-
1
-
5
-
1
1
1
-
-
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-
-
-
-
-
1
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
736942
Zinsser
Citrate synthase from the live ...
Fasciola hepatica
Parasitol. Res.
112
2413-2417
2013
-
-
1
-
-
-
-
1
-
-
1
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
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-
-
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1
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1
-
-
-
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-
1
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1
1
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1
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-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
737105
Zhu
Engineering of acetate recycli ...
Bacillus subtilis
PLoS ONE
8
e60659
2013
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
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-
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1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719138
van Ooyen
Improved L-lysine production w ...
Corynebacterium glutamicum
Biotechnol. Bioeng.
109
2070-2081
2012
-
-
-
-
-
-
-
-
-
-
-
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1
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1
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1
-
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719429
Moore
Contribution of inter-subunit ...
Pyrococcus furiosus
Extremophiles
15
327-336
2011
-
-
1
-
2
-
-
-
-
-
1
-
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1
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1
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1
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1
1
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1
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2
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1
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1
1
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1
1
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720082
van Ooyen
Citrate synthase in Corynebact ...
Corynebacterium glutamicum
J. Biotechnol.
154
140-148
2011
-
-
-
-
1
-
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-
-
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1
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2
2
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1
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1
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2
2
-
1
-
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720215
Min
Differential expression of cit ...
Aspergillus nidulans, Aspergillus nidulans FGSC A26
J. Microbiol.
48
188-198
2010
-
-
1
-
-
-
-
-
2
-
1
-
-
3
-
-
-
-
-
2
-
-
2
1
-
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-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
2
-
-
2
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
701519
Larson
Structure of pig heart citrate ...
Sus scrofa
Acta Crystallogr. Sect. F
65
430-434
2009
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702337
Kurz
The partial substrate dethiaac ...
Thermoplasma acidophilum
Biochemistry
48
7878-7891
2009
-
-
1
-
8
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
4
-
-
-
1
-
-
8
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
6
-
-
-
4
-
-
-
-
-
-
704320
Mittal
CcpC-dependent regulation of c ...
Listeria monocytogenes
J. Bacteriol.
191
862-872
2009
-
-
1
-
-
-
-
-
-
-
-
-
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1
-
-
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-
-
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705562
Buch
Enhanced citric acid biosynthe ...
Escherichia coli
Microbiology
155
2620-2629
2009
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705651
Han
One novel mitochondrial citrat ...
Oryza sativa
Mol. Biotechnol.
42
299-305
2009
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706384
Deng
Overexpression of Citrus junos ...
Citrus junos
Planta
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355-365
2009
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1
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684713
Lin
Suppression of metabolic defec ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae MMY011
Arch. Biochem. Biophys.
474
205-212
2008
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2
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686229
Fields
Function of muscle-type lactat ...
Amblyrhynchus cristatus, Iguana iguana
Comp. Biochem. Physiol. B Biochem. Mol. Biol.
150
62-73
2008
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2
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686703
Qiu
ArHsp22, a developmentally reg ...
Saccharomyces cerevisiae
FEBS J.
275
3556-3566
2008
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1
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1
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689524
Barone
Bacterial citrate synthase exp ...
Pseudomonas aeruginosa
Plant Cell Rep.
27
893-901
2008
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1
-
1
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1
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689614
Sienkiewicz-Porzucek
Mild reductions in mitochondri ...
Solanum lycopersicum, Solanum lycopersicum Moneymaker
Plant Physiol.
147
115-127
2008
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1
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703601
Mailloux
A novel metabolic network lead ...
Pseudomonas fluorescens
Extremophiles
12
451-459
2008
-
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674333
Li
Re-citrate synthase from Clost ...
Clostridium kluyveri
J. Bacteriol.
189
4299-4304
2007
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684681
Graybill
Functional comparison of citra ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
465
26-37
2007
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1
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689928
Snow
Essential dynamics sampling st ...
Escherichia coli
Proteins
67
325-337
2007
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1
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672091
Francois
Structure of a NADH-insensitiv ...
Acetobacter aceti
Biochemistry
45
13487-13499
2006
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1
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4
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672744
De Maeseneire
Metabolic characterisation of ...
Escherichia coli
Biotechnol. Lett.
28
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2006
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657610
Bond
Characterization of citrate sy ...
Geobacter sulfurreducens
Appl. Environ. Microbiol.
71
3858-3865
2005
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4
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1
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1
4
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2
1
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4
1
1
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1
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660134
Pracharoenwattana
Arabidopsis peroxisomal citrat ...
Arabidopsis thaliana
Plant Cell
17
2037-2048
2005
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4
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4
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671987
Kurz
Photophysics of tryptophan flu ...
Thermoplasma acidophilum
Biochemistry
44
1394-1413
2005
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1
4
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11
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11
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11
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1
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11
-
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2
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674243
Suksomtip
Citrate synthase mutants of Ag ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970
J. Bacteriol.
187
4844-4852
2005
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1
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20
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676102
Grzemski
Probing the Sinorhizobium meli ...
Sinorhizobium meliloti
Mol. Plant Microbe Interact.
18
134-141
2005
-
1
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1
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1
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1
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1
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-
656929
Iannetta
Identification, cloning and ex ...
Fragaria x ananassa
Physiol. Plant.
121
15-26
2004
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1
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2
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1
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1
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-
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-
-
-
488088
Nordberg Karlsson
Citrate synthase from Thermus ...
Thermus aquaticus
Extremophiles
7
9-16
2003
-
-
1
-
-
-
-
-
-
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1
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1
1
2
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1
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1
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1
1
2
-
1
-
-
-
-
-
-
-
-
-
-
-
659254
Stokell
Probing the roles of key resid ...
Escherichia coli
J. Biol. Chem.
278
35435-35443
2003
-
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1
3
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2
19
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-
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2
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1
1
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20
-
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20
-
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1
3
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2
20
19
-
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-
1
1
-
-
-
20
-
-
-
-
-
-
-
-
-
-
488086
Ruprich-Robert
Lack of mitochondrial citrate ...
Podospora anserina, Saccharomyces cerevisiae
EMBO J.
21
6440-6451
2002
-
-
1
-
2
-
-
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3
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5
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2
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1
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2
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3
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2
-
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-
-
-
-
-
-
-
-
-
-
-
-
488087
Karlsson
Rhodothermus marinus: a thermo ...
Rhodothermus marinus
Extremophiles
6
51-56
2002
-
-
-
-
-
-
-
6
-
-
5
-
-
1
-
-
1
-
-
-
2
-
2
2
2
1
1
3
-
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-
-
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-
-
-
-
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-
6
-
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5
-
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1
-
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2
-
2
2
2
1
1
3
-
-
-
-
-
-
-
-
-
-
658613
Bell
Stepwise adaptations of citrat ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Eur. J. Biochem.
269
6250-6260
2002
-
-
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1
-
-
-
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4
-
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2
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1
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-
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488084
Gerike
Cold-active citrate synthase: ...
Arthrobacter sp., Arthrobacter sp. DS2-3R, Pyrococcus furiosus
Protein Eng.
14
655-661
2001
-
-
1
-
8
-
-
15
-
-
-
-
-
3
-
-
-
-
-
-
-
-
5
-
-
-
2
8
2
-
-
-
-
-
-
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-
1
-
-
8
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
2
8
2
-
-
-
-
-
-
-
-
-
488085
Nguyen
Comparative analysis of foldin ...
Escherichia coli
Biochemistry
40
13177-13187
2001
-
-
-
1
-
-
2
-
-
-
1
-
-
3
-
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1
1
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-
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1
1
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-
1
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2
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1
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1
-
-
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-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657705
Sadka
-
Comparative analysis of mitoch ...
Citrus limettioides, Citrus limon, Citrus sinensis
Aust. J. Plant Physiol.
28
383-390
2001
-
-
-
-
-
-
-
-
-
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3
-
3
-
-
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3
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3
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3
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3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488074
Ruijter
Properties of Aspergillus nige ...
Aspergillus niger, Aspergillus niger N400 / DSM 2466 / ATCC 9029 / CBS 120.49
FEMS Microbiol. Lett.
184
35-40
2000
-
-
1
-
-
-
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2
2
-
3
2
-
2
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1
-
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1
1
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4
1
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1
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1
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2
2
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3
2
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1
-
1
1
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
488075
Kocabiyik
The effect of valine substitut ...
Thermoplasma acidophilum
Biochem. Biophys. Res. Commun.
275
460-465
2000
2
-
1
-
1
-
2
4
-
-
-
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1
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1
-
-
-
2
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1
1
1
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2
2
1
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2
-
1
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1
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2
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4
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1
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-
2
-
1
1
1
-
2
2
1
-
-
-
-
-
-
-
-
-
488076
Arnott
Thermostability and thermoacti ...
Pyrococcus furiosus, Thermoplasma acidophilum
J. Mol. Biol.
304
657-668
2000
-
-
2
2
4
-
-
4
-
-
-
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2
-
-
2
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-
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2
4
8
2
2
-
2
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2
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2
4
-
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4
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2
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-
-
-
2
4
8
2
2
-
2
-
-
-
-
-
-
-
-
-
488077
Pettersson
Kinetics of the coupled reacti ...
Saccharomyces cerevisiae
Eur. J. Biochem.
267
5041-5046
2000
-
-
1
-
1
-
-
-
-
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1
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1
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1
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1
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1
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1
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1
-
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-
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-
-
-
-
488078
Tabrett
Biochemical controls of citrat ...
Cicer arietinum, Mesorhizobium ciceri, Mesorhizobium ciceri CC 1192
Arch. Microbiol.
173
42-48
2000
1
-
-
-
-
2
20
2
-
2
2
-
-
3
-
-
2
-
-
2
2
1
3
-
-
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-
-
1
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1
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-
1
-
-
-
-
-
2
-
20
1
2
-
2
2
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2
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2
2
1
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
488080
Salomon
-
Effects of temperature on the ...
Idotea baltica, Idotea emarginata
Comp. Biochem. Physiol. B
125B
71-81
2000
-
-
-
-
-
-
-
1
-
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-
2
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2
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2
-
-
-
-
-
-
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-
-
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-
-
-
-
488081
Koyama
Overexpression of mitochondria ...
Arabidopsis thaliana, Arabidopsis thaliana WS, Daucus carota
Plant Cell Physiol.
41
1030-1037
2000
-
2
1
-
1
-
-
-
4
-
-
3
-
7
-
-
-
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1
4
-
4
-
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3
1
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2
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3
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1
4
-
4
-
-
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488082
Kurz
Kinetics and mechanism of the ...
Sus scrofa, Thermoplasma acidophilum
Biochemistry
39
2283-2296
2000
-
-
1
-
-
-
-
-
-
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1
-
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2
-
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1
2
-
1
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2
-
2
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6
1
-
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1
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-
-
-
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1
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1
-
1
-
-
2
-
2
-
-
6
1
-
-
-
-
-
-
-
-
-
488089
Brock
Methylcitrate synthase from As ...
Aspergillus nidulans
Mol. Microbiol.
35
961-973
2000
-
-
1
-
-
-
-
3
-
-
1
-
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1
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1
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2
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1
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3
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1
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1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488072
Haggie
Mitochondrial citrate synthase ...
Saccharomyces cerevisiae
J. Biol. Chem.
274
3941-3945
1999
-
-
1
-
-
-
-
-
1
-
1
1
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2
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1
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1
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2
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1
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1
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1
1
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1
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1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727213
Connaris
Expression, reactivation, and ...
Haloferax volcanii, Haloferax volcanii DSM 3757
Biotechnol. Bioeng.
64
38-45
1999
-
-
1
-
-
-
1
4
-
-
1
-
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2
-
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1
-
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1
1
2
1
1
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1
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1
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1
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4
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1
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1
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-
1
1
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
724945
Connaris
Cloning and overexpression in ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Extremophiles
2
61-66
1998
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
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-
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2
1
1
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1
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1
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1
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1
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1
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2
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
488045
Russell
The crystal structure of citra ...
Pyrococcus furiosus
Biochemistry
36
9983-9994
1997
-
-
-
1
-
-
-
-
-
-
1
-
-
1
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1
1
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1
1
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3
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1
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1
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1
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1
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
488050
Henderson
Poly-3-hydroxybutyrate product ...
Cupriavidus necator
Arch. Microbiol.
168
486-492
1997
-
1
-
-
-
-
-
2
-
-
2
1
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1
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1
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2
1
1
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1
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1
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2
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1
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1
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1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488051
Sievers
Purification and properties of ...
Komagataeibacter europaeus
FEMS Microbiol. Lett.
146
53-58
1997
1
-
-
-
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-
1
2
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3
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1
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1
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1
1
1
1
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1
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1
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-
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1
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2
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3
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1
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1
1
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
488059
Lee
Characterization of citrate sy ...
Drosophila melanogaster
Mol. Cells
7
599-604
1997
-
-
-
-
-
-
5
2
-
-
3
-
-
1
-
-
1
1
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1
-
1
1
1
-
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
5
4
2
-
-
3
-
-
-
-
1
-
-
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488070
Belova
Purification and characterizat ...
Methylorubrum extorquens
Biochemistry
62
71-76
1997
-
-
-
-
-
-
5
2
-
1
2
1
-
1
-
-
1
-
-
-
1
1
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
2
-
1
2
1
-
-
-
1
-
-
1
1
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488067
Kocabryik
The effect of C43 mutation on ...
Thermoplasma acidophilum
Biochem. Biophys. Res. Commun.
224
224-228
1996
-
-
1
-
1
-
-
2
-
-
-
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1
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-
1
-
-
-
2
-
1
-
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1
-
1
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-
1
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-
1
-
-
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2
-
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1
-
-
2
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
488028
Rault-Leonardon
Azotobacter vinelandii citrate ...
Azotobacter vinelandii
Biochemistry
34
257-263
1995
-
-
-
-
-
-
1
2
-
1
4
1
-
1
-
-
1
-
-
-
-
-
2
1
1
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-
3
1
-
-
1
2
-
-
-
-
-
1
-
-
-
-
1
2
2
-
1
4
1
-
-
-
1
-
-
-
-
2
1
1
-
-
3
1
-
-
-
-
-
-
-
-
-
488054
Landschuetze
Mitochondrial citrate synthase ...
Solanum tuberosum
Planta
196
756-764
1995
-
-
1
-
-
-
-
-
2
-
1
2
-
5
-
-
-
-
-
5
2
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
2
-
-
-
-
-
5
2
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488057
Muir
Citrate synthase from the hype ...
Pyrococcus furiosus
Protein Eng.
8
583-592
1995
-
-
1
-
-
-
-
2
-
2
3
-
-
1
-
-
1
-
-
-
2
-
1
1
1
-
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
2
3
-
-
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-
1
-
-
2
-
1
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
488062
Mitchell
-
Purification and characterizat ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa 8602
Biochem. J.
309
507-511
1995
1
-
-
-
-
-
3
4
-
-
6
-
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6
-
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1
-
-
-
6
1
4
3
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
4
-
-
6
-
-
-
-
1
-
-
6
1
4
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
488063
Kojima
Citrate synthase purified from ...
Tetrahymena pyriformis, Tetrahymena pyriformis W
J. Biochem.
118
189-195
1995
-
-
-
-
-
-
2
2
1
1
1
2
-
7
-
-
1
-
-
-
1
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
1
1
1
2
-
-
-
1
-
-
1
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
488064
Takeda
Polymerization of highly purif ...
Tetrahymena pyriformis
J. Biochem.
117
869-874
1995
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
1
-
-
-
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488071
Asai
-
Purification and properties of ...
Nitrosomonas sp., Nitrosomonas sp. TK794
J. Ferment. Bioeng.
79
67-69
1995
-
-
-
-
-
-
8
2
-
1
2
-
-
2
-
-
1
-
-
-
1
-
2
1
1
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
8
-
2
-
1
2
-
-
-
-
1
-
-
1
-
2
1
1
-
1
-
3
-
1
-
-
-
-
-
-
-
488060
Pereira
Active site mutants of Escheri ...
Escherichia coli
J. Biol. Chem.
269
412-417
1994
-
-
-
-
9
-
1
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
9
-
-
1
2
3
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488061
Sandor
Cooperation between enzyme and ...
Saccharomyces cerevisiae
J. Biol. Chem.
269
29609-29612
1994
-
-
-
-
-
-
-
-
5
-
-
1
-
3
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-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488069
Molgat
Chimeric allosteric citrate sy ...
Acinetobacter calcoaceticus subsp. anitratus, Escherichia coli
Arch. Biochem. Biophys.
298
238-246
1992
-
-
2
-
2
-
2
4
-
2
2
-
-
2
-
-
2
-
-
-
6
-
2
2
-
-
-
4
1
-
-
-
-
-
-
-
-
2
-
-
2
-
-
2
-
4
-
2
2
-
-
-
-
2
-
-
6
-
2
2
-
-
-
4
1
-
-
-
-
-
-
-
-
-
724900
Lill
Conversion, by limited proteol ...
Saccharolobus solfataricus, Saccharolobus solfataricus P1, Saccharolobus solfataricus P2
Eur. J. Biochem.
208
459-466
1992
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-
-
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1
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2
2
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14
-
-
1
-
-
-
-
1
4
1
1
-
-
-
1
-
-
-
2
1
-
-
-
-
-
-
1
-
-
3
4
4
-
-
-
-
-
-
-
2
-
-
-
1
4
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
488066
James
Citrate synthase from Halofera ...
Haloferax volcanii
Biochem. Soc. Trans.
20
12S
1991
-
-
-
-
-
-
-
-
-
-
2
1
-
1
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-
1
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-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488068
Kispal
Studies on yeast peroxisomal c ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
286
132-137
1991
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-
1
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1
7
5
5
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2
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2
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-
1
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-
1
1
1
1
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1
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-
-
-
-
-
1
-
-
-
1
-
7
-
5
5
-
2
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1
-
-
1
1
1
1
-
-
-
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1
-
-
-
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-
-
-
-
488053
Unger
Purification and characterizat ...
Pisum sativum
Plant Physiol.
89
719-723
1989
1
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2
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2
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1
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1
1
-
1
-
-
-
-
-
-
-
-
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-
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1
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2
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1
-
1
1
-
1
-
-
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-
-
-
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-
-
-
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-
-
-
-
488047
Sautter
Glyoxysomal citrate synthase f ...
Citrullus lanatus subsp. vulgaris
Planta
173
289-297
1988
-
-
1
-
-
-
2
-
2
-
1
1
-
1
-
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1
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-
1
1
-
2
1
1
-
-
-
1
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-
2
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1
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-
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-
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2
2
-
2
-
1
1
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-
-
1
-
1
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488048
Loehlein-Wehrhahn
Purification and properties of ...
Saccharolobus solfataricus
Biol. Chem. Hoppe-Seyler
369
109-113
1988
-
-
-
-
-
-
2
2
-
-
2
-
-
1
-
-
1
-
-
-
1
1
1
1
-
-
2
-
-
-
-
-
3
-
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-
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-
-
-
-
-
2
3
2
-
-
2
-
-
-
-
1
-
-
1
1
1
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
719023
Loehlein-Werhahn
Studies on the citryl-CoA-depe ...
Saccharolobus solfataricus
Biol. Chem. Hoppe-Seyler
369
417-424
1988
-
-
-
-
-
-
-
-
-
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1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488049
Smith
-
Citrate synthase from the ther ...
Sulfolobus acidocaldarius, Sulfolobus acidocaldarius 98-3 / DSM 639, Thermoplasma acidophilum
FEBS Lett.
225
277-281
1987
-
-
-
-
-
-
-
-
-
-
5
-
-
4
-
-
2
-
-
-
2
-
3
4
2
-
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-
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5
-
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2
-
-
2
-
3
4
2
-
-
-
-
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-
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-
-
-
-
-
488044
Otto
-
Regulatory and molecular prope ...
Arthrobacter sp., Arthrobacter sp. 2B2, Bacillus subtilis, Bacillus subtilis S2A1, Hyphomicrobium sp., Methylophilus methylotrophus
FEMS Microbiol. Lett.
34
191-194
1986
-
-
-
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4
3
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7
6
-
6
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-
-
-
-
-
12
-
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-
-
-
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-
1
-
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-
-
-
-
-
-
4
1
3
-
-
7
6
-
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-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662550
Mitchell
Molecular size diversity of ci ...
Brevundimonas diminuta, Brevundimonas diminuta D 1032, Comamonas testosteroni, Comamonas testosteroni D 1047, Delftia acidovorans, Delftia acidovorans D 1870, Pelomonas saccharophila, Pelomonas saccharophila D 1021, Pseudomonas aeruginosa, Pseudomonas alcaligenes, Pseudomonas alcaligenes D 123, Pseudomonas chlororaphis, Pseudomonas chlororaphis D 302, Pseudomonas fluorescens, Pseudomonas fluorescens D 1086, Pseudomonas putida, Pseudomonas putida NCIB 8296, Pseudomonas stutzeri, Pseudomonas stutzeri D 1022, Stenotrophomonas maltophilia, Stenotrophomonas maltophilia D 144
J. Gen. Microbiol.
132
737-742
1986
-
-
-
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15
-
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11
-
-
21
-
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-
-
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-
-
15
-
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-
11
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
799
Danson
-
Citric acid cycle enzymes of t ...
Haloarcula vallismortis, Halobacterium salinarum, Haloferax volcanii, Methanosarcina barkeri, Natronobacterium gregoryi, Natronococcus occultus, Natronomonas pharaonis, Sulfolobus acidocaldarius, Thermoplasma acidophilum
FEBS Lett.
179
120-124
1985
-
-
-
-
-
-
25
14
-
-
8
-
-
9
-
-
-
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-
7
-
10
-
7
-
-
-
-
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-
19
-
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-
-
-
-
-
25
19
14
-
-
8
-
-
-
-
-
-
-
7
-
10
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
488043
Grossebuter
-
Partial purification and prope ...
Sulfolobus acidocaldarius, Sulfolobus acidocaldarius 98-3 / DSM 639, Thermoplasma acidophilum, Thermoplasma acidophilum 122-1B3 / ATCC 27658
Syst. Appl. Microbiol.
6
119-124
1985
-
-
-
-
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-
6
4
-
-
2
-
-
5
-
-
2
-
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-
2
-
4
-
-
-
2
-
-
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-
-
6
-
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-
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6
6
4
-
-
2
-
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-
2
-
-
2
-
4
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
488040
Robinson
Citrate synthase of Escherichi ...
Escherichia coli
FEBS Lett.
154
51-54
1983
-
-
1
-
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2
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3
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1
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1
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1
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1
1
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1
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2
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3
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1
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1
-
1
1
-
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-
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-
-
-
-
-
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-
-
-
-
488041
Robinson
Citrate synthase from a gram-p ...
Bacillus megaterium, Bacillus megaterium D101
Biochem. J.
213
53-59
1983
-
-
-
-
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1
7
2
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2
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2
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1
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1
2
4
1
1
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1
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1
-
7
-
2
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-
2
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1
-
-
1
2
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488037
Okabayashi
Partial purification and prope ...
Heliocidaris crassispina
J. Biochem.
85
1061-1066
1979
-
-
-
-
-
-
15
2
-
-
1
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-
1
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1
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1
1
1
1
-
1
-
2
-
1
-
1
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-
-
-
-
-
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-
15
-
2
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-
1
-
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1
-
1
1
1
1
-
1
-
2
-
1
-
1
-
-
-
-
-
-
-
488038
Alejandre
-
Characteristics of citrate syn ...
Agave americana
Z. Pflanzenphysiol.
94
85-93
1979
-
-
-
-
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-
1
2
1
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1
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1
1
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1
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1
1
1
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1
1
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1
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1
1
2
1
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-
1
1
-
1
-
1
1
1
-
1
1
-
-
-
-
-
-
-
-
488039
Iredale
-
Properties of citrate synthase ...
Pisum sativum
Phytochemistry
18
1057
1979
-
-
-
-
-
-
7
2
1
-
-
1
-
1
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1
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2
-
1
-
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-
1
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1
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-
-
-
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7
1
2
1
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-
1
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-
1
-
-
2
-
1
-
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-
1
-
-
-
-
-
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-
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-
488035
Higa
Purification and some properti ...
Pseudomonas sp.
Can. J. Microbiol.
24
215-221
1978
1
-
-
-
-
1
6
4
-
2
3
-
-
1
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-
1
-
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-
1
1
1
2
1
-
-
-
1
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-
1
-
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-
1
-
-
1
-
-
1
-
6
-
4
-
2
3
-
-
-
-
1
-
-
1
1
1
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488036
Weitzman
Anomalous citrate synthase fro ...
Thermus aquaticus
J. Gen. Microbiol.
106
383-386
1978
-
-
-
-
-
-
2
2
-
-
2
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1
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1
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1
-
1
-
1
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2
-
2
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-
2
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-
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-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
488034
Porter
Partial purification and chara ...
Dictyostelium discoideum, Dictyostelium discoideum NC-4 / ATCC 24697
Arch. Biochem. Biophys.
181
155-163
1977
1
-
-
-
-
2
7
2
-
1
1
2
-
3
-
-
1
1
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-
1
2
4
-
1
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
2
-
7
1
2
-
1
1
2
-
-
-
1
-
-
1
2
4
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
488033
Higa
Some properties of the citrate ...
Halobacterium salinarum
Biochem. J.
147
267-274
1975
-
-
-
-
-
1
1
3
-
5
-
-
-
1
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1
-
-
-
2
1
1
-
1
-
1
-
1
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-
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-
1
-
1
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3
-
5
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1
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-
2
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
488030
Johnson
Bacterial citrate synthases: p ...
Acinetobacter calcoaceticus subsp. anitratus, Azotobacter vinelandii, Azotobacter vinelandii OP, Bacillus subtilis, Bacillus subtilis HS2A2
Biochim. Biophys. Acta
350
336-353
1974
2
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-
1
18
4
-
2
5
5
-
8
-
-
3
3
-
-
4
-
10
2
3
-
-
2
-
-
-
-
2
-
-
2
-
-
-
-
-
1
-
18
2
4
-
2
5
5
-
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-
3
-
-
4
-
10
2
3
-
-
2
-
-
-
-
-
-
-
-
-
-
488029
Mahlen
Purification and some properti ...
Talaromyces trachyspermus
Eur. J. Biochem.
29
60-66
1972
-
-
-
-
-
1
4
5
-
1
1
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1
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-
1
-
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1
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1
-
1
-
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-
1
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-
1
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-
-
-
-
-
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-
1
-
4
1
5
-
1
1
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-
-
1
-
-
1
-
1
-
1
-
-
-
1
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-
-
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-