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Literature summary for 2.3.3.16 extracted from

  • Tabrett, C.A.; Copeland, L.
    Biochemical controls of citrate synthase in chickpea bacteroids (2000), Arch. Microbiol., 173, 42-48.
    View publication on PubMed
Search for more literature for 2.3.3.16

Activating Compound

Activating Compound Comment Organism Structure
Urea isocitrate, (R)-3-hydroxybutyrate, malonate, pyruvate, acetoacetyl have no effect Mesorhizobium ciceri

General Stability

General Stability Organism
freeze-drying results in 70% loss of activity Mesorhizobium ciceri
KCl stabilizes Mesorhizobium ciceri

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate 30% inhibition at 1 mM Cicer arietinum
2-oxoglutarate no inhibition Mesorhizobium ciceri
acetyl-CoA no inhibition Mesorhizobium ciceri
ADP no inhibition Mesorhizobium ciceri
AMP no inhibition Mesorhizobium ciceri
ATP 30% inhibition at 1 mM Cicer arietinum
ATP no inhibition Mesorhizobium ciceri
CaCl2 50% inhibition at 10 mM, not reversible by KCl Mesorhizobium ciceri
cAMP no inhibition Mesorhizobium ciceri
citrate 50% inhibition at 2.5 mM Mesorhizobium ciceri
CoA 50% inhibition at 0.2 mM Mesorhizobium ciceri
L-malate no inhibition Mesorhizobium ciceri
MgCl2 50% inhibition at 7 mM, not reversible by KCl Mesorhizobium ciceri
additional information isocitrate, (R)-3-hydroxybutyrate, malonate, pyruvate, acetoacetyl, NaCl, NH4Cl, CsCl, and RbCl have no effect Mesorhizobium ciceri
NaCl no effect Mesorhizobium ciceri
NADH no effect Cicer arietinum
NADH complete inhibition at 1 mM, completely reversible by NAD+ Mesorhizobium ciceri
NADP+ no inhibition Mesorhizobium ciceri
NADPH no inhibition Mesorhizobium ciceri
oxaloacetate no inhibition Mesorhizobium ciceri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.088
-
 acetyl-CoA in presence of 100 mM KCl Mesorhizobium ciceri
0.174
-
 acetyl-CoA in absence of KCl Mesorhizobium ciceri

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 40% activation at 50 mM, 30% activation at 100 mM Mesorhizobium ciceri
additional information NaCl, NH4Cl, CsCl, and RbCl have no effect Mesorhizobium ciceri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 gel filtration Cicer arietinum
228000
-
 gel filtration Mesorhizobium ciceri

Organism

Organism UniProt Comment Textmining
Cicer arietinum
-
-
-
Mesorhizobium ciceri
-
 bacteroids formed in symbiosis with Cicer arietinum plants
-
Mesorhizobium ciceri CC 1192
-
 bacteroids formed in symbiosis with Cicer arietinum plants
-

Purification (Commentary)

Purification (Comment) Organism
partial Cicer arietinum
partial Mesorhizobium ciceri

Source Tissue

Source Tissue Comment Organism Textmining
root nodule
-
 Cicer arietinum
-
root nodule bacteroids formed in symbiosis with Cicer arietinum plants Mesorhizobium ciceri
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.87
-
 partially purified enzyme Cicer arietinum
1.25
-
 partially purified enzyme Mesorhizobium ciceri

Storage Stability

Storage Stability Organism
4°C, 25 mM Tris-HCl, pH 7.7, loss of 80% activity within 3 days, 50 mM KCl protects Mesorhizobium ciceri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
 Cicer arietinum citrate + CoA
-
 ?
acetyl-CoA + oxaloacetate + H2O
-
 Mesorhizobium ciceri citrate + CoA
-
 ?
acetyl-CoA + oxaloacetate + H2O
-
 Mesorhizobium ciceri CC 1192 citrate + CoA
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 7.8
-
 Mesorhizobium ciceri

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.023
-
 NADH
-
 Mesorhizobium ciceri