Cloned (Comment) | Organism |
---|---|
functional expression of peroxisomal isozyme CS II in citrate synthase deficient Escherichia coli mutant and wild-type Escherichia coli strain | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
peroxisomal enzyme protected by oxaloacetate | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Saccharomyces cerevisiae | |
AMP | only CS II | Saccharomyces cerevisiae | |
ATP | strong inhibition | Saccharomyces cerevisiae | |
additional information | no inhibition by NAD+ | Saccharomyces cerevisiae | |
NADH | 51% inhibition of peroxisomal isozyme, 12% inhibition of mitochondrial isozyme at 5 mM | Saccharomyces cerevisiae | |
NADP+ | only CS II slightly | Saccharomyces cerevisiae | |
NADPH | 40% inhibition by 5 mM, both isoenzymes | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
oxaloacetate | mitochondrial isozyme CS I | Saccharomyces cerevisiae | |
0.004 | - |
acetyl-CoA | mitochondrial isozyme CS I | Saccharomyces cerevisiae | |
0.006 | 0.007 | oxaloacetate | native and recombinant peroxisomal isozyme CS II | Saccharomyces cerevisiae | |
0.011 | - |
acetyl-CoA | native peroxisomal isozyme CS II | Saccharomyces cerevisiae | |
0.013 | 0.014 | acetyl-CoA | recombinant peroxisomal isozyme CS II | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | 45% of CS II is bound to mitochondrial inner membrane and 2% of CS I | Saccharomyces cerevisiae | 5743 | - |
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
peroxisome | - |
Saccharomyces cerevisiae | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, CS II, SDS-PAGE | Saccharomyces cerevisiae |
100000 | - |
gel filtration | Saccharomyces cerevisiae |
100000 | - |
CS II | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
peroxisomal isozyme CS II and mitochondrial isozyme CS I | - |
Purification (Comment) | Organism |
---|---|
recombinant peroxisomal isozyme CS II from E. coli | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
70 | - |
purified recombinant isozyme CS II | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, Tris-HCl buffer, pH 7.4, 50% glycerol, 1 month | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | - |
Saccharomyces cerevisiae | citrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, CS II, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
both mitochondrial and peroxisomal enzymes | Saccharomyces cerevisiae |