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Literature summary for 2.3.3.16 extracted from
- Glyoxysomal citrate synthase from watermelon cotyledons: immunochemical localization and heterologous translation in Xenopus oocytes (1988), Planta, 173, 289-297.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| in vitro translation of glyoxysomal isozyme, heterologous expression in Xenopus laevis oocytes | Citrullus lanatus subsp. vulgaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | inactivation of the glyoxysomal isozyme, half-life: approx. 1 min, not mitochondrial isozyme | Citrullus lanatus subsp. vulgaris |  |
| ATP | - |
Citrullus lanatus subsp. vulgaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| glyoxysome | - |
Citrullus lanatus subsp. vulgaris | 9514 | - |
| mitochondrion | - |
Citrullus lanatus subsp. vulgaris | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
x * 48000, glyoxysomal isozyme, SDS-PAGE | Citrullus lanatus subsp. vulgaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | Citrullus lanatus subsp. vulgaris | key enzyme of glyoxylate cycle in fat-storing seedlings | citrate + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrullus lanatus subsp. vulgaris | - |
water melon, mitochondrial and glyoxysomal isoenzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| mitochondrial isozyme partially, glyoxysomal isozyme to homogeneity | Citrullus lanatus subsp. vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Citrullus lanatus subsp. vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.764 | - |
purified glyoxysomal isozyme | Citrullus lanatus subsp. vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | - |
Citrullus lanatus subsp. vulgaris | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | key enzyme of glyoxylate cycle in fat-storing seedlings | Citrullus lanatus subsp. vulgaris | citrate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000, glyoxysomal isozyme, SDS-PAGE | Citrullus lanatus subsp. vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
enzyme assay at | Citrullus lanatus subsp. vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
enzyme assay at | Citrullus lanatus subsp. vulgaris |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.33 | - |
ATP | mitochondrial isozyme | Citrullus lanatus subsp. vulgaris | |
| 2.6 | - |
ATP | glyoxysomal isozyme | Citrullus lanatus subsp. vulgaris | |
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