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Literature summary for 2.3.3.16 extracted from

  • Grossebuter, W.; Goerisch, H.
    Partial purification and properties of citrate synthase from the thermoacidophilic archaebacteria Thermoplasma acidophilum and Sulfolobus acidocaldarius (1985), Syst. Appl. Microbiol., 6, 119-124.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
ATP competitive against acetyl-CoA Sulfolobus acidocaldarius
ATP competitive against acetyl-CoA Thermoplasma acidophilum
NADH competitive against acetyl-CoA Sulfolobus acidocaldarius
NADH competitive against acetyl-CoA Thermoplasma acidophilum
NADPH competitive against acetyl-CoA Sulfolobus acidocaldarius
NADPH competitive against acetyl-CoA Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0041
-
oxaloacetate
-
Thermoplasma acidophilum
0.006
-
acetyl-CoA
-
Thermoplasma acidophilum
0.01
-
acetyl-CoA
-
Sulfolobus acidocaldarius
0.026
-
oxaloacetate
-
Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
gel filtration Thermoplasma acidophilum
73000
-
gel filtration Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
-
-
-
Sulfolobus acidocaldarius 98-3 / DSM 639
-
-
-
Thermoplasma acidophilum
-
-
-
Thermoplasma acidophilum 122-1B3 / ATCC 27658
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Sulfolobus acidocaldarius
partial Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14
-
partially purified enzyme Sulfolobus acidocaldarius
18.8
-
partially purified enzyme Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
Sulfolobus acidocaldarius citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O
-
Thermoplasma acidophilum citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O
-
Sulfolobus acidocaldarius 98-3 / DSM 639 citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O
-
Thermoplasma acidophilum 122-1B3 / ATCC 27658 citrate + CoA
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
90% of activity after 10 min Sulfolobus acidocaldarius
80
-
65% of activity retained after 10 min Thermoplasma acidophilum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.4
-
ATP
-
Sulfolobus acidocaldarius
0.5
-
ATP
-
Thermoplasma acidophilum
0.8
-
NADPH
-
Sulfolobus acidocaldarius
4.4
-
NADH
-
Sulfolobus acidocaldarius
4.9
-
NADPH
-
Thermoplasma acidophilum
10.4
-
NADH
-
Thermoplasma acidophilum