Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.3.16 extracted from

  • Robinson, M.S.; Easom, R.A.; Danson, M.J.; Weitzman, P.D.J.
    Citrate synthase of Escherichia coli. Characterization of the enzyme from a plasmid-cloned gene and amplification of the intracellular levels (1983), FEBS Lett., 154, 51-54.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate
-
Escherichia coli
NADH allosteric Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
6 * 44000, SDS-PAGE, 6 * 49000, guanidine-HCl gel filtration Escherichia coli
49000
-
6 * 44000, SDS-PAGE, 6 * 49000, guanidine-HCl gel filtration Escherichia coli
269000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein expressed from plasmid Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
62.6
-
purified enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
Escherichia coli citrate + CoA
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 44000, SDS-PAGE, 6 * 49000, guanidine-HCl gel filtration Escherichia coli