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Literature summary for 2.3.3.16 extracted from
- Partial purification and properties of citrate synthase from sea urchin eggs (1979), J. Biochem., 85, 1061-1066.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Heliocidaris crassispina |  |
| AMP | - |
Heliocidaris crassispina | |
| ATP | 44% inhibition at 5 mM | Heliocidaris crassispina | |
| CaCl2 | 29% inhibition at 10 mM | Heliocidaris crassispina | |
| Cations | monovalent and divalent | Heliocidaris crassispina | |
| CTP | poor | Heliocidaris crassispina | |
| GTP | poor | Heliocidaris crassispina | |
| iodoacetamide | 10% inhibition at 0.002 mM | Heliocidaris crassispina | |
| KCl | 9% inhibition at 50 mM | Heliocidaris crassispina | |
| MgCl2 | 32% inhibition at 10 mM | Heliocidaris crassispina | |
| MnCl2 | 56% inhibition at 10 mM | Heliocidaris crassispina | |
| NaCl | 15% inhibition at 50 mM | Heliocidaris crassispina | |
| p-chloromercuribenzoate | 6% loss at 0.002 mM | Heliocidaris crassispina | |
| TTP | poor | Heliocidaris crassispina | |
| UTP | poor | Heliocidaris crassispina | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0032 | - |
oxaloacetate | - |
Heliocidaris crassispina | |
| 0.033 | - |
acetyl-CoA | - |
Heliocidaris crassispina | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
gel filtration | Heliocidaris crassispina |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Heliocidaris crassispina | - |
sea urchin | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Heliocidaris crassispina |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| egg | - |
Heliocidaris crassispina | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1190 | - |
partially purified enzyme | Heliocidaris crassispina |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, phosphate buffer, pH 7.4, 20% loss of activity after 2 weeks | Heliocidaris crassispina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | - |
Heliocidaris crassispina | citrate + CoA | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
enzyme assay at | Heliocidaris crassispina |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
oxaloacetate protects against heat denaturation | Heliocidaris crassispina |
| 50 | - |
in presence of oxaloacetate 70% of activity retained after 10 min, in absence of oxaloacetate complete loss of activity, acetyl-CoA does not protect | Heliocidaris crassispina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Heliocidaris crassispina |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
unstable below | Heliocidaris crassispina |
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