Literature summary for 2.3.3.14 extracted from

Andi, B.; West, A.H.; Cook, P.F.
Stabilization and characterization of histidine-tagged homocitrate synthase from Saccharomyces cerevisiae (2004), Arch. Biochem. Biophys., 421, 243-254.

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General Stability

General Stability	Organism
the purified enzyme can be stabilized using a combination of guanidine hydrochloride, alpha-cyclodextrin and ammonium sulfate	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.024	-	acetyl-CoA	pH 7.5	Saccharomyces cerevisiae
1.3	-	2-oxoglutarate	pH 7.5	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
His-tagged enzyme	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + H2O + 2-oxoglutarate	-	Saccharomyces cerevisiae	2-hydroxybutane-1,2,4-tricarboxylate + CoA	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.617	-	acetyl-CoA	-	Saccharomyces cerevisiae
0.617	-	2-oxoglutarate	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)