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Literature summary for 2.3.3.13 extracted from

  • Yoshida, A.; Kosono, S.; Nishiyama, M.
    Characterization of two 2-isopropylmalate synthase homologs from Thermus thermophilus HB27 (2018), Biochem. Biophys. Res. Commun., 501, 465-470 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
isoleucine inhibited by leucine and isoleucine. Leucine is a more effective inhibitor Thermus thermophilus
leucine inhibited by leucine and isoleucine. Leucine is a more effective inhibitor Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
acetyl-CoA pH 8.0, 50°C Thermus thermophilus
0.054
-
3-methyl-2-oxobutanoate pH 8.0, 50°C Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Thermus thermophilus enzyme of the branched-chain amino acid biosynthetic gene cluster. The enzyme is involved in biosynthesis of leucine and isoleucine (2S)-2-isopropylmalate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q72JC9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Thermus thermophilus (2S)-2-isopropylmalate + CoA
-
?
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O enzyme of the branched-chain amino acid biosynthetic gene cluster. The enzyme is involved in biosynthesis of leucine and isoleucine Thermus thermophilus (2S)-2-isopropylmalate + CoA
-
?

Synonyms

Synonyms Comment Organism
IPMS
-
Thermus thermophilus
TT_C0849
-
Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
3-methyl-2-oxobutanoate pH 8.0, 50°C Thermus thermophilus
1.7
-
acetyl-CoA pH 8.0, 50°C Thermus thermophilus

General Information

General Information Comment Organism
metabolism enzyme of the branched-chain amino acid biosynthetic gene cluster. The enzyme is involved in biosynthesis of leucine and isoleucine Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
22
-
3-methyl-2-oxobutanoate pH 8.0, 50°C Thermus thermophilus
73
-
acetyl-CoA pH 8.0, 50°C Thermus thermophilus