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Literature summary for 2.3.3.13 extracted from

  • Zhang, Z.; Wu, J.; Lin, W.; Wang, J.; Yan, H.; Zhao, W.; Ma, J.; Ding, J.; Zhang, P.; Zhao, G.P.
    Subdomain II of alpha-isopropylmalate synthase is essential for activity: inferring a mechanism of feedback inhibition (2014), J. Biol. Chem., 289, 27966-27978.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Leptospira biflexa

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.5), 0.8 mM sodium formate, and 30% (w/v) PEG 2000 monomethyl ether Leptospira biflexa

General Stability

General Stability Organism
partial deletion of the regulatory domain of isoform IPMS1 results in a loss of about 50% of the catalytic activity Leptospira biflexa

Inhibitors

Inhibitors Comment Organism Structure
L-leucine
-
Leptospira biflexa
Zn2+ Zn2+ slightly inhibits the enzyme activity Leptospira biflexa

Metals/Ions

Metals/Ions Comment Organism Structure
K+ an excess amount of 50 mM K+ is included in the standard enzymatic assays. Only isoform IPMS1 is coactivated by monovalent metal ions like K+ Leptospira biflexa
Mn2+ most effective activator, an excess amount 2 mM Mn2+ is included in the standard enzymatic assays Leptospira biflexa
NH4+ only isoform IPMS1 is coactivated by monovalent metal ions like NH4+ Leptospira biflexa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Leptospira biflexa
-
(2S)-2-isopropylmalate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Leptospira biflexa B0SN40
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Leptospira biflexa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Leptospira biflexa (2S)-2-isopropylmalate + CoA
-
?
acetyl-CoA + 3-methyl-2-oxobutyrate + H2O
-
Leptospira biflexa ?
-
?
acetyl-CoA + 3-methyl-2-oxoisovalerate + H2O
-
Leptospira biflexa ?
-
?
acetyl-CoA + 3-methyl-2-oxovalerate + H2O
-
Leptospira biflexa ?
-
?
additional information isoform IPMS2 shows no activity with 4-methylthio-2-oxobutyrate or 2-oxohexanoate Leptospira biflexa ?
-
?

Synonyms

Synonyms Comment Organism
Alpha-isopropylmalate synthase
-
Leptospira biflexa
IPMS1 long enzyme form Leptospira biflexa
IPMS2 short enzyme form Leptospira biflexa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
isoform IPMS1 Leptospira biflexa
50
-
isoform IPMS2 Leptospira biflexa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
the stability of isoform IPMS2 isremarkably decreased when the temperature exceeds 45°C and is higher than that of isoform IPMS1 at 37°C Leptospira biflexa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Leptospira biflexa