Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Leptospira biflexa |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.5), 0.8 mM sodium formate, and 30% (w/v) PEG 2000 monomethyl ether | Leptospira biflexa |
General Stability | Organism |
---|---|
partial deletion of the regulatory domain of isoform IPMS1 results in a loss of about 50% of the catalytic activity | Leptospira biflexa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-leucine | - |
Leptospira biflexa | |
Zn2+ | Zn2+ slightly inhibits the enzyme activity | Leptospira biflexa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | an excess amount of 50 mM K+ is included in the standard enzymatic assays. Only isoform IPMS1 is coactivated by monovalent metal ions like K+ | Leptospira biflexa | |
Mn2+ | most effective activator, an excess amount 2 mM Mn2+ is included in the standard enzymatic assays | Leptospira biflexa | |
NH4+ | only isoform IPMS1 is coactivated by monovalent metal ions like NH4+ | Leptospira biflexa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Leptospira biflexa | - |
(2S)-2-isopropylmalate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leptospira biflexa | B0SN40 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Leptospira biflexa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Leptospira biflexa | (2S)-2-isopropylmalate + CoA | - |
? | |
acetyl-CoA + 3-methyl-2-oxobutyrate + H2O | - |
Leptospira biflexa | ? | - |
? | |
acetyl-CoA + 3-methyl-2-oxoisovalerate + H2O | - |
Leptospira biflexa | ? | - |
? | |
acetyl-CoA + 3-methyl-2-oxovalerate + H2O | - |
Leptospira biflexa | ? | - |
? | |
additional information | isoform IPMS2 shows no activity with 4-methylthio-2-oxobutyrate or 2-oxohexanoate | Leptospira biflexa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Alpha-isopropylmalate synthase | - |
Leptospira biflexa |
IPMS1 | long enzyme form | Leptospira biflexa |
IPMS2 | short enzyme form | Leptospira biflexa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
isoform IPMS1 | Leptospira biflexa |
50 | - |
isoform IPMS2 | Leptospira biflexa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the stability of isoform IPMS2 isremarkably decreased when the temperature exceeds 45°C and is higher than that of isoform IPMS1 at 37°C | Leptospira biflexa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Leptospira biflexa |