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Literature summary for 2.3.3.13 extracted from

  • Hunter, M.F.; Parker, E.J.
    Modifying the determinants of alpha-ketoacid substrate selectivity in Mycobacterium tuberculosis alpha-isopropylmalate synthase (2014), FEBS Lett., 588, 1603-1607.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Star cells Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
H167A the mutant shows reduced activity compared to the wild type enzyme Mycobacterium tuberculosis
L143A the mutant shows reduced activity compared to the wild type enzyme Mycobacterium tuberculosis
L143N the mutant shows reduced activity compared to the wild type enzyme Mycobacterium tuberculosis
N250A the mutant shows reduced activity and is able to use (3RS)-3-methyl-2-oxovalerate and 4-methyl-2-oxovalerate as substrates compared to the wild type enzyme Mycobacterium tuberculosis
S216G the mutant shows reduced activity and is able to use (3RS)-3-methyl-2-oxovalerate and 4-methyl-2-oxovalerate as substrates compared to the wild type enzyme Mycobacterium tuberculosis
Y169A the mutant shows reduced activity compared to the wild type enzyme Mycobacterium tuberculosis
Y169H the mutant shows reduced activity compared to the wild type enzyme Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Mycobacterium tuberculosis
-
(2S)-2-isopropylmalate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WQB3
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap affinity column chromatography and gel Sephacryl 26/60 filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 2-oxobutyrate + H2O weak substrate Mycobacterium tuberculosis ?
-
?
acetyl-CoA + 2-oxovalerate + H2O weak substrate Mycobacterium tuberculosis ?
-
?
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Mycobacterium tuberculosis (2S)-2-isopropylmalate + CoA
-
?
acetyl-CoA + pyruvate + H2O weak substrate Mycobacterium tuberculosis ?
-
?
additional information no activity with (3RS)-3-methyl-2-oxovalerate and 4-methyl-2-oxovalerate Mycobacterium tuberculosis ?
-
?

Synonyms

Synonyms Comment Organism
Alpha-isopropylmalate synthase
-
Mycobacterium tuberculosis
IPMS
-
Mycobacterium tuberculosis

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the first step of leucine biosynthesis Mycobacterium tuberculosis