Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Star cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
H167A | the mutant shows reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
L143A | the mutant shows reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
L143N | the mutant shows reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
N250A | the mutant shows reduced activity and is able to use (3RS)-3-methyl-2-oxovalerate and 4-methyl-2-oxovalerate as substrates compared to the wild type enzyme | Mycobacterium tuberculosis |
S216G | the mutant shows reduced activity and is able to use (3RS)-3-methyl-2-oxovalerate and 4-methyl-2-oxovalerate as substrates compared to the wild type enzyme | Mycobacterium tuberculosis |
Y169A | the mutant shows reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
Y169H | the mutant shows reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Mycobacterium tuberculosis | - |
(2S)-2-isopropylmalate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WQB3 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap affinity column chromatography and gel Sephacryl 26/60 filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 2-oxobutyrate + H2O | weak substrate | Mycobacterium tuberculosis | ? | - |
? | |
acetyl-CoA + 2-oxovalerate + H2O | weak substrate | Mycobacterium tuberculosis | ? | - |
? | |
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Mycobacterium tuberculosis | (2S)-2-isopropylmalate + CoA | - |
? | |
acetyl-CoA + pyruvate + H2O | weak substrate | Mycobacterium tuberculosis | ? | - |
? | |
additional information | no activity with (3RS)-3-methyl-2-oxovalerate and 4-methyl-2-oxovalerate | Mycobacterium tuberculosis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Alpha-isopropylmalate synthase | - |
Mycobacterium tuberculosis |
IPMS | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the first step of leucine biosynthesis | Mycobacterium tuberculosis |