Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-leucine | isoform Leu4, but not Leu9, is feedback inhibited by L-leucine | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
2 * 60000, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Saccharomyces cerevisiae | - |
(2S)-2-isopropylmalate + CoA | - |
? | |
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Saccharomyces cerevisiae BY4741 | - |
(2S)-2-isopropylmalate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae BY4741 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q sepharose column chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Saccharomyces cerevisiae | (2S)-2-isopropylmalate + CoA | - |
? | |
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Saccharomyces cerevisiae BY4741 | (2S)-2-isopropylmalate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 60000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
alpha-IPM synthase | - |
Saccharomyces cerevisiae |
alpha-IPMS | - |
Saccharomyces cerevisiae |
Alpha-isopropylmalate synthase | - |
Saccharomyces cerevisiae |
LEU4 | isoform | Saccharomyces cerevisiae |
LEU9 | isoform | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.28 | - |
3-methyl-2-oxobutanoate | isoform Leu9, at pH 7.5 and 30°C | Saccharomyces cerevisiae | |
13.79 | - |
3-methyl-2-oxobutanoate | isoform Leu4, at pH 7.5 and 30°C | Saccharomyces cerevisiae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.03 | - |
at pH 7.5 and 30°C | Saccharomyces cerevisiae | L-leucine |