Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.3.13 extracted from

  • Lopez, G.; Quezada, H.; Duhne, M.; Gonzalez, J.; Lezama, M.; El-Hafidi, M.; Colon, M.; Martinez de la Escalera, X.; Flores-Villegas, M.C.; Scazzocchio, C.; DeLuna, A.; Gonzalez, A.
    Diversification of paralogous alpha-isopropylmalate synthases by modulation of feedback control and hetero-oligomerization in Saccharomyces cerevisiae (2015), Eukaryot. Cell, 14, 564-577.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
L-leucine isoform Leu4, but not Leu9, is feedback inhibited by L-leucine Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Saccharomyces cerevisiae 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
2 * 60000, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Saccharomyces cerevisiae
-
(2S)-2-isopropylmalate + CoA
-
?
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Saccharomyces cerevisiae BY4741
-
(2S)-2-isopropylmalate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae BY4741
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q sepharose column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Saccharomyces cerevisiae (2S)-2-isopropylmalate + CoA
-
?
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Saccharomyces cerevisiae BY4741 (2S)-2-isopropylmalate + CoA
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 60000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
alpha-IPM synthase
-
Saccharomyces cerevisiae
alpha-IPMS
-
Saccharomyces cerevisiae
Alpha-isopropylmalate synthase
-
Saccharomyces cerevisiae
LEU4 isoform Saccharomyces cerevisiae
LEU9 isoform Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.28
-
3-methyl-2-oxobutanoate isoform Leu9, at pH 7.5 and 30°C Saccharomyces cerevisiae
13.79
-
3-methyl-2-oxobutanoate isoform Leu4, at pH 7.5 and 30°C Saccharomyces cerevisiae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.03
-
at pH 7.5 and 30°C Saccharomyces cerevisiae L-leucine