Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, 96% decrease in activity | Methanocaldococcus jannaschii | |
L-leucine | partially inhibited by L-leucine in a V-type manner | Methanocaldococcus jannaschii | |
MgCl2 | 20 mM decreases activity | Methanocaldococcus jannaschii | |
MnCl2 | 20 mM decreases activity | Methanocaldococcus jannaschii | |
ZnCl2 | 20 mM decreases activity | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
acetyl-CoA | pH 7.8, 37°C | Methanocaldococcus jannaschii | |
0.09 | - |
3-methyl-2-oxobutanoate | pH 7.8, 37°C | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | 100 mM, activity is increased 1.5fold | Methanocaldococcus jannaschii | |
additional information | the enzyme is active in the absence of exogenous monovalent or divalent metals. Addition of 1 mM EDTA results in a 96% decrease in activity, consistent with the requirement for divalent metals and suggesting that the enzyme is copurified with a divalent metal | Methanocaldococcus jannaschii | |
NaCl | 100 mM, activity is increased 1.5fold | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
230000 | - |
gel filtration | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Methanocaldococcus jannaschii | the hydrolytic step is rate-determining | (2S)-2-isopropylmalate + CoA | - |
? | |
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Methanocaldococcus jannaschii DSM 2661 | the hydrolytic step is rate-determining | (2S)-2-isopropylmalate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58595 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q58595 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Storage Stability | Organism |
---|---|
-20°C, concentrated protein stored in 20% glycerol | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | the hydrolytic step is rate-determining | Methanocaldococcus jannaschii | (2S)-2-isopropylmalate + CoA | - |
? | |
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | the hydrolytic step is rate-determining | Methanocaldococcus jannaschii DSM 2661 | (2S)-2-isopropylmalate + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IPMS | - |
Methanocaldococcus jannaschii |
leuA | - |
Methanocaldococcus jannaschii |
Mj1195 | locus name | Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
the enzyme retains full activity after incubation in water for 15 min | Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
330 | - |
acetyl-CoA | pH 7.8, 37°C | Methanocaldococcus jannaschii | |
330 | - |
3-methyl-2-oxobutanoate | pH 7.8, 37°C | Methanocaldococcus jannaschii | |
450 | - |
acetyl-CoA | pH 7.8, 55°C | Methanocaldococcus jannaschii | |
450 | - |
3-methyl-2-oxobutanoate | pH 7.8, 55°C | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Methanocaldococcus jannaschii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00016 | - |
L-leucine | pH 7.8, 37°C | Methanocaldococcus jannaschii |