Protein Variants | Comment | Organism |
---|---|---|
D563A | the enzyme is more sensitive to L-leucine inhibition compared to the wild type enzyme | Mycobacterium tuberculosis |
D563N | the enzyme is more sensitive to L-leucine inhibition compared to the wild type enzyme | Mycobacterium tuberculosis |
D564A | the enzyme is less sensitive to L-leucine inhibition compared to the wild type enzyme | Mycobacterium tuberculosis |
H540A | the enzyme is less sensitive to L-leucine inhibition compared to the wild type enzyme | Mycobacterium tuberculosis |
M559A | the enzyme is less sensitive to L-leucine inhibition compared to the wild type enzyme | Mycobacterium tuberculosis |
Q566A | the enzyme is more sensitive to L-leucine inhibition compared to the wild type enzyme | Mycobacterium tuberculosis |
S560A | inactive | Mycobacterium tuberculosis |
S560W | the mutation does not affect the activity of the enzyme, but the enzyme is less sensitive to L-leucine inhibition compared to the wild type enzyme | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-isoleucine | - |
Mycobacterium tuberculosis | |
L-leucine | slow-onset, allosteric inhibition by L-leucine | Mycobacterium tuberculosis | |
L-methionine | - |
Mycobacterium tuberculosis | |
L-norleucine | micromolar inhibitor | Mycobacterium tuberculosis | |
L-norvaline | micromolar inhibitor | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme requires a divalent metal for activity and is activated by monovalent cations | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Mycobacterium tuberculosis | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
alpha-isopropylmalate synthase I | - |
Mycobacterium tuberculosis |
IPMS | - |
Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
L-leucine | mutant enzyme D563N, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
0.003 | - |
L-leucine | mutant enzyme D563A, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
0.0077 | - |
L-leucine | mutant enzyme Q566A, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
0.012 | - |
L-leucine | wild type enzyme, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
0.023 | - |
L-leucine | mutant enzyme H540A, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
0.024 | - |
L-leucine | mutant enzyme D564A, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
0.051 | - |
L-leucine | mutant enzyme S560W, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
0.155 | - |
L-leucine | mutant enzyme M559A, pH and temperature not specified in the publication | Mycobacterium tuberculosis |