KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
2-oxo-3-methylbutanoate | pH 7.4, 25°C | Mycobacterium tuberculosis | |
0.05 | - |
crotonyl-CoA | hydrolysis, absence of MgCl2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
0.057 | - |
acetyl-CoA | cosubstrate 2-oxo-butanoate, pH 7.4, 25°C | Mycobacterium tuberculosis | |
0.074 | - |
acetyl-CoA | cosubstrate pyruvate, pH 7.4, 25°C | Mycobacterium tuberculosis | |
0.136 | - |
acetyl-CoA | cosubstrate 2-oxo-3-methylbutanoate, pH 7.4, 25°C | Mycobacterium tuberculosis | |
0.16 | - |
acetyl-CoA | hydrolysis, presence of MgCl2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
0.22 | - |
propionyl-CoA | hydrolysis, absence of MgCl2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
0.279 | - |
acetyl-CoA | cosubstrate 2-oxo-n-pentanoate, pH 7.4, 25°C | Mycobacterium tuberculosis | |
0.37 | - |
acetyl-CoA | hydrolysis, absence of MgCl2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
0.41 | - |
2-oxo-n-pentanoate | pH 7.4, 25°C | Mycobacterium tuberculosis | |
0.86 | - |
2-oxo-butanoate | pH 7.4, 25°C | Mycobacterium tuberculosis | |
0.95 | - |
crotonyl-CoA | hydrolysis, presence of MgCl2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
9.5 | - |
pyruvate | pH 7.4, 25°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA | nonrapid equilibrium bi-bi kinetic mechanism, with a preferred pathway to the ternary complex. Hydrolysis of alpha-isopropylmalyl-CoA is direct, without the formation of a cyclic anhydride intermediate | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Mycobacterium tuberculosis | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? | |
2-oxo-butanoate + acetyl-CoA + H2O | - |
Mycobacterium tuberculosis | 2-ethyl-2-hydroxysuccinic acid + CoA | - |
? | |
2-oxo-n-pentanoate + acetyl-CoA + H2O | - |
Mycobacterium tuberculosis | 2-hydroxy-2-propylsuccinic acid + CoA | - |
? | |
acetyl-CoA + H2O | - |
Mycobacterium tuberculosis | acetate + CoA | - |
? | |
crotonyl-CoA + H2O | - |
Mycobacterium tuberculosis | crotonate + CoA | - |
? | |
propionyl-CoA + H2O | - |
Mycobacterium tuberculosis | propanoate + CoA | - |
? | |
pyruvate + acetyl-CoA + H2O | - |
Mycobacterium tuberculosis | 2-hydroxy-2-methylsuccinic acid + CoA | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
acetyl-CoA | hydrolysis, presence of MgCl2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
0.04 | - |
propionyl-CoA | hydrolysis, absence of MgCl2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
0.04 | - |
crotonyl-CoA | hydrolysis, presence of MgCl2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
0.05 | - |
crotonyl-CoA | hydrolysis, absence of MgCl2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
0.06 | - |
acetyl-CoA | hydrolysis, absence of MgCl2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
2.1 | - |
acetyl-CoA | cosubstrate 2-oxo-3-methylbutanoate, pH 7.4, 25°C | Mycobacterium tuberculosis | |
3.5 | - |
2-oxo-3-methylbutanoate | pH 7.4, 25°C | Mycobacterium tuberculosis | |
4.5 | - |
acetyl-CoA | cosubstrate pyruvate, pH 7.4, 25°C | Mycobacterium tuberculosis | |
5 | - |
acetyl-CoA | cosubstrate 2-oxo-n-pentanoate, pH 7.4, 25°C | Mycobacterium tuberculosis | |
6.1 | - |
pyruvate | pH 7.4, 25°C | Mycobacterium tuberculosis | |
7 | - |
2-oxo-n-pentanoate | pH 7.4, 25°C | Mycobacterium tuberculosis | |
7.6 | - |
acetyl-CoA | cosubstrate 2-oxo-butanoate, pH 7.4, 25°C | Mycobacterium tuberculosis | |
10.7 | - |
2-oxo-butanoate | pH 7.4, 25°C | Mycobacterium tuberculosis |