Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.3.13 extracted from

  • de Carvalho, L.P.; Blanchard, J.S.
    Kinetic analysis of the effects of monovalent cations and divalent metals on the activity of Mycobacterium tuberculosis alpha-isopropylmalate synthase (2006), Arch. Biochem. Biophys., 451, 141-148.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 50% inhibition at 0.205 mM Mycobacterium tuberculosis
Zn2+ 50% inhibition at 0.031 mM Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of Mn2+ Mycobacterium tuberculosis
0.0022
-
2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of Co2+ Mycobacterium tuberculosis
0.012
-
2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of Mg2+ Mycobacterium tuberculosis
0.016
-
2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of K+ Mycobacterium tuberculosis
0.019
-
2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of Rb+ Mycobacterium tuberculosis
0.037
-
acetyl-CoA pH 7.4, 25°C, presence of Co2+ Mycobacterium tuberculosis
0.05
-
acetyl-CoA pH 7.4, 25°C, presence of Mn2+ Mycobacterium tuberculosis
0.103
-
acetyl-CoA pH 7.4, 25°C, presence of K+ Mycobacterium tuberculosis
0.12
-
acetyl-CoA pH 7.4, 25°C, presence of Rb+ Mycobacterium tuberculosis
0.138
-
acetyl-CoA pH 7.4, 25°C, presence of Mg2+ Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ ratio kcat/Kact 0.052 per s and M. Detailed kinetic analysis Mycobacterium tuberculosis
K+ K+ is the likely physiological allosteric activator Mycobacterium tuberculosis
Mg2+ and Mn2+, best activators, ratio kcat/Kact 0.014 per s and M. Detailed kinetic analysis Mycobacterium tuberculosis
Mn2+ and Mg2+, best activators, ratio kcat/Kact 0.034 per s and M. Detailed kinetic analysis Mycobacterium tuberculosis
Rb+ activation Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-3-methylbutanoate + acetyl-CoA + H2O
-
Mycobacterium tuberculosis 3-hydroxy-4-methyl-3-carboxypentanoate + CoA
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.77
-
2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of Co2+ Mycobacterium tuberculosis
1.69
-
2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of Mn2+ Mycobacterium tuberculosis
2.9
-
2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of Rb+ Mycobacterium tuberculosis
3.2
-
2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of K+ Mycobacterium tuberculosis
3.5
-
2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of Mg2+ Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00037
-
Zn2+ pH 7.5, 25°C Mycobacterium tuberculosis
0.0025
-
Ca2+ pH 7.5, 25°C Mycobacterium tuberculosis