Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 50% inhibition at 0.205 mM | Mycobacterium tuberculosis | |
Zn2+ | 50% inhibition at 0.031 mM | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
2-oxo-3-methylbutanoate | pH 7.4, 25°C, presence of Mn2+ | Mycobacterium tuberculosis | |
0.0022 | - |
2-oxo-3-methylbutanoate | pH 7.4, 25°C, presence of Co2+ | Mycobacterium tuberculosis | |
0.012 | - |
2-oxo-3-methylbutanoate | pH 7.4, 25°C, presence of Mg2+ | Mycobacterium tuberculosis | |
0.016 | - |
2-oxo-3-methylbutanoate | pH 7.4, 25°C, presence of K+ | Mycobacterium tuberculosis | |
0.019 | - |
2-oxo-3-methylbutanoate | pH 7.4, 25°C, presence of Rb+ | Mycobacterium tuberculosis | |
0.037 | - |
acetyl-CoA | pH 7.4, 25°C, presence of Co2+ | Mycobacterium tuberculosis | |
0.05 | - |
acetyl-CoA | pH 7.4, 25°C, presence of Mn2+ | Mycobacterium tuberculosis | |
0.103 | - |
acetyl-CoA | pH 7.4, 25°C, presence of K+ | Mycobacterium tuberculosis | |
0.12 | - |
acetyl-CoA | pH 7.4, 25°C, presence of Rb+ | Mycobacterium tuberculosis | |
0.138 | - |
acetyl-CoA | pH 7.4, 25°C, presence of Mg2+ | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | ratio kcat/Kact 0.052 per s and M. Detailed kinetic analysis | Mycobacterium tuberculosis | |
K+ | K+ is the likely physiological allosteric activator | Mycobacterium tuberculosis | |
Mg2+ | and Mn2+, best activators, ratio kcat/Kact 0.014 per s and M. Detailed kinetic analysis | Mycobacterium tuberculosis | |
Mn2+ | and Mg2+, best activators, ratio kcat/Kact 0.034 per s and M. Detailed kinetic analysis | Mycobacterium tuberculosis | |
Rb+ | activation | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Mycobacterium tuberculosis | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.77 | - |
2-oxo-3-methylbutanoate | pH 7.4, 25°C, presence of Co2+ | Mycobacterium tuberculosis | |
1.69 | - |
2-oxo-3-methylbutanoate | pH 7.4, 25°C, presence of Mn2+ | Mycobacterium tuberculosis | |
2.9 | - |
2-oxo-3-methylbutanoate | pH 7.4, 25°C, presence of Rb+ | Mycobacterium tuberculosis | |
3.2 | - |
2-oxo-3-methylbutanoate | pH 7.4, 25°C, presence of K+ | Mycobacterium tuberculosis | |
3.5 | - |
2-oxo-3-methylbutanoate | pH 7.4, 25°C, presence of Mg2+ | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00037 | - |
Zn2+ | pH 7.5, 25°C | Mycobacterium tuberculosis | |
0.0025 | - |
Ca2+ | pH 7.5, 25°C | Mycobacterium tuberculosis |