Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5',5',5'-trifluoroleucine | reversed by acetyl-CoA | Neurospora crassa | |
Br- | above 0.02 M | Neurospora crassa | |
DL-azaleucine | 10 mM, at pH 6.5 | Neurospora crassa | |
DL-fluoroleucine | at pH 7.5 | Neurospora crassa | |
F- | above 0.02 M | Neurospora crassa | |
L-Phe | 10 mM, at pH 6.5 | Neurospora crassa | |
L-Val | 10 mM, at pH 6.5 | Neurospora crassa | |
leucine | at pH 7.5 | Neurospora crassa | |
Zn2+ | 0.1 mM | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
2-oxo-3-methylbutanoate | - |
Neurospora crassa | |
0.0245 | - |
acetyl-CoA | pH 7.5 | Neurospora crassa | |
0.028 | - |
acetyl-CoA | pH 7.0 | Neurospora crassa | |
0.035 | - |
acetyl-CoA | pH 6.6 | Neurospora crassa | |
2.1 | - |
2-oxo-butanoate | - |
Neurospora crassa | |
5.8 | - |
pyruvate | - |
Neurospora crassa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Neurospora crassa | |
Li+ | stimulates at low concentrations | Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | Neurospora crassa | first enzyme in biosynthesis of L-Leu | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Neurospora crassa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Neurospora crassa |
Storage Stability | Organism |
---|---|
-20°C, phosphate leucine buffer, little loss of activity | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Neurospora crassa | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | first enzyme in biosynthesis of L-Leu | Neurospora crassa | ? | - |
? | |
2-oxo-butanoate + acetyl-CoA + H2O | - |
Neurospora crassa | 2-ethyl-2-hydroxysuccinic acid + CoA | - |
? | |
pyruvate + acetyl-CoA + H2O | - |
Neurospora crassa | 2-hydroxy-2-methylsuccinic acid + CoA | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal stability at pH 6.5 increases as a function of Leu concentration. The protection afforded by Leu is greatest at pH 6.5, less at pH 7.5, and is entirely lost at pH 8.3 | Neurospora crassa |
49 | - |
7 min, 90% loss of activity in absence of Leu, about 50% loss of activity after 15 min in presence of 0.2 mM Leu | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Neurospora crassa |