Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Oxo-isohexanoate | - |
Cupriavidus necator | |
leucine | at low concentrations of Leu the inhibition mechanism is of the competitive type with respect to substrate acetyl-CoA and of the non-competitive type with respect to 2-oxo-3-methylbutanoate; L-Leu | Cupriavidus necator |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Cupriavidus necator | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
44 | - |
pH: 7.5, temperature optimum increased to 45°C in the presence of 2 mM valine and to 48°C in the presence of leucine | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Cupriavidus necator |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
leucine | pH 7.35, acetyl-CoA varied | Cupriavidus necator | |
0.002 | - |
leucine | pH 7.6, acetyl-CoA varied | Cupriavidus necator | |
0.0025 | - |
leucine | pH 8.1, acetyl-CoA varied | Cupriavidus necator | |
0.1 | - |
leucine | pH 7.6, 2-oxo-3-methylbutanoate varied | Cupriavidus necator | |
0.1 | - |
2-Oxo-isohexanoate | with respect to 2-oxo-3-methylbutanoate | Cupriavidus necator | |
0.2 | - |
leucine | pH 8.1, 2-oxo-3-methylbutanoate varied | Cupriavidus necator | |
0.2 | - |
2-Oxo-isohexanoate | with respect to acetyl-CoA | Cupriavidus necator |