Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Oxo-isohexanoate | - |
Cupriavidus necator | |
CoA | competitive against both 2-oxo-3-methylbutanoate and acetyl-CoA | Cupriavidus necator | |
isopropylmalate | competitive against both 2-oxo-3-methylbutanoate and acetyl-CoA | Cupriavidus necator | |
leucine | L-Leu | Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
2-oxo-3-methylbutanoate | with acetyl-CoA as cosubstrate | Cupriavidus necator | |
0.075 | - |
acetyl-CoA | - |
Cupriavidus necator | |
0.1 | - |
propanoyl-CoA | - |
Cupriavidus necator | |
0.4 | - |
butanoyl-CoA | - |
Cupriavidus necator | |
0.4 | - |
2-oxo-n-pentanoate | with acetyl-CoA as cosubstrate | Cupriavidus necator | |
0.5 | - |
malonyl-CoA | - |
Cupriavidus necator | |
0.9 | - |
pentanoyl-CoA | - |
Cupriavidus necator | |
1.8 | - |
2-oxobutanoate | with acetyl-CoA as cosubstrate | Cupriavidus necator | |
5 | - |
2-oxo-3-methylbutanoyl-methylester | with acetyl-CoA as cosubstrate | Cupriavidus necator | |
10 | - |
pyruvate | with acetyl-CoA as cosubstrate | Cupriavidus necator |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Cupriavidus necator H 16 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Cupriavidus necator | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Cupriavidus necator H 16 | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? | |
2-oxo-3-methylbutanoate + butanoyl-CoA + H2O | - |
Cupriavidus necator | 3-ethyl-2-hydroxy-2-isopropylsuccinic acid + CoA | - |
? | |
2-oxo-3-methylbutanoate + malonyl-CoA + H2O | - |
Cupriavidus necator | 2-hydroxy-3-metylbutane-11,2-tricarboxyl acid + CoA | - |
? | |
2-oxo-3-methylbutanoate + propanoyl-CoA + H2O | - |
Cupriavidus necator | 2-hydroxy-2-isopropyl-3-methylsuccinic acid + CoA | - |
? | |
2-oxo-3-methylbutanoate + propanoyl-CoA + H2O | - |
Cupriavidus necator H 16 | 2-hydroxy-2-isopropyl-3-methylsuccinic acid + CoA | - |
? | |
2-oxo-3-methylbutanoate + valeryl-CoA + H2O | - |
Cupriavidus necator | 2-hydroxy-2-isopropyl-3-propylsuccinic acid + CoA | - |
? | |
2-oxo-3-methylbutanoyl-methylester + H2O | - |
Cupriavidus necator | 3-hydroxy-3-(methoxycarbonyl)-4-methylpentanoic acid + CoA | - |
? | |
2-oxo-butanoate + acetyl-CoA + H2O | - |
Cupriavidus necator | 2-ethyl-2-hydroxysuccinic acid + CoA | - |
? | |
2-oxo-butanoate + acetyl-CoA + H2O | - |
Cupriavidus necator H 16 | 2-ethyl-2-hydroxysuccinic acid + CoA | - |
? | |
2-oxo-n-pentanoate + acetyl-CoA + H2O | - |
Cupriavidus necator | 2-hydroxy-2-propylsuccinic acid + CoA | - |
? | |
2-oxo-n-pentanoate + acetyl-CoA + H2O | - |
Cupriavidus necator H 16 | 2-hydroxy-2-propylsuccinic acid + CoA | - |
? | |
pyruvate + acetyl-CoA + H2O | - |
Cupriavidus necator | 2-hydroxy-2-methylsuccinic acid + CoA | - |
? | |
pyruvate + acetyl-CoA + H2O | - |
Cupriavidus necator H 16 | 2-hydroxy-2-methylsuccinic acid + CoA | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
isopropylmalate | with respect to 2-oxo-3-methylbutanoate at a substrate concentration of 0.07 mM acetyl-CoA | Cupriavidus necator | |
0.9 | - |
isopropylmalate | with respect to acetyl-CoA at a substrate concentration of 0.375 mM 2-oxo-3-methylbutanoate | Cupriavidus necator | |
4 | - |
isopropylmalate | with respect to acetyl-CoA at a substrate concentration of 0.75 mM 2-oxo-3-methylbutanoate | Cupriavidus necator |