Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | dialysis at an initial concentration of 50 mM reduces the zinc content by more than 80%, complete loss of activity, restored by addition of Zn2+, Mn2+, Fe2+, Co2+, or Cd2+ | Saccharomyces cerevisiae | |
leucine | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km of enzyme with and without Zn2+ or Mn2+ | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | contains approximately 4 gatoms of zinc per dimer of 130000 Da | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | i.e. alpha-isopropylmalate | ? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
leucine | Ki with and without Zn2+ or Mn2+ | Saccharomyces cerevisiae |