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Literature summary for 2.3.3.13 extracted from

  • Bode, R.; Birnbaum, D.
    Some properties of the leucine-biosynthesizing enzymes from Candida maltosa (1991), J. Basic Microbiol., 31, 21-26.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
Candida maltosa
Cu2+
-
Candida maltosa
Hg2+
-
Candida maltosa
K+ at high concentrations, stimulation at lower concentrations Candida maltosa
leucine competitive with respect to 2-oxo-3-methylbutanoate, non-competitive with respect to acetyl-CoA Candida maltosa
Zn2+
-
Candida maltosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Candida maltosa
0.064
-
acetyl-CoA
-
Candida maltosa
0.57
-
2-oxo-3-methylbutanoate
-
Candida maltosa

Metals/Ions

Metals/Ions Comment Organism Structure
K+ strongly dependent on the presence of monovalent cations, K+ is most effective Candida maltosa
K+ Km: 80 mM, inhibitory at higher concentrations Candida maltosa
Li+ can partially replace K+ in activation Candida maltosa
Rb+ can partially replace Rb+ in activation Candida maltosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxo-3-methylbutanoate + acetyl-CoA + H2O Candida maltosa
-
?
-
?

Organism

Organism UniProt Comment Textmining
Candida maltosa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-3-methylbutanoate + acetyl-CoA + H2O
-
Candida maltosa 3-hydroxy-4-methyl-3-carboxypentanoate + CoA
-
?
2-oxo-3-methylbutanoate + acetyl-CoA + H2O
-
Candida maltosa ?
-
?
2-oxo-butanoate + acetyl-CoA + H2O
-
Candida maltosa 2-ethyl-2-hydroxysuccinic acid + CoA
-
?
pyruvate + acetyl-CoA + H2O
-
Candida maltosa 2-hydroxy-2-methylsuccinic acid + CoA
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.8
-
Candida maltosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.81
-
leucine competitive, with respect to 2-oxo-3-methylbutanoate Candida maltosa
1.3
-
leucine non-competitive, with respect to acetyl-CoA Candida maltosa
230
-
K+ at high concentration Candida maltosa