Application | Comment | Organism |
---|---|---|
analysis | a visible wavelength spectrophotometric assay suitable for high-throughput screening of 3-hydroxy-3-methylglutaryl-CoA synthase is established | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
into the vector pET-HSyn for expression in Escherichia coli cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-benzyl-9-(3,6-dioxocyclohexa-1,4-dienyl)-2,3,7,8-tetrahydro-6H-pyrido[1,2-a]pyrazine-1,4-dione | - |
Homo sapiens | |
3-chloropropionyl-CoA | - |
Homo sapiens | |
3-hydroxy-3-methylglutaryl-CoA | - |
Homo sapiens | |
acetyloxy(phenyl)mercury | phenyl mercuric acetate | Homo sapiens | |
diethylcarbamothioylsulfanyl N,N-diethylcarbamodithioate | disulfiram | Homo sapiens | |
hymeglusin | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
acetyl-CoA | comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A412 nm standard assay | Homo sapiens | |
0.076 | - |
acetyl-CoA | comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A300 nm standard assay | Homo sapiens | |
0.084 | - |
acetyl-CoA | comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A412 nm microplate assay | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + acetoacetyl-CoA | Homo sapiens | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + acetoacetyl-CoA | - |
Homo sapiens | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxy-3-methylglutaryl-CoA synthase | - |
Homo sapiens |
HMG-CoA | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
activity assay, A412 nm assay | Homo sapiens |
30 | - |
activity assay, A300 nm assay | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
3-hydroxy-3-methylglutaryl-CoA | comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A300 nm standard assay | Homo sapiens | |
0.0015 | - |
3-hydroxy-3-methylglutaryl-CoA | comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A412 nm standard assay | Homo sapiens | |
0.0031 | - |
3-hydroxy-3-methylglutaryl-CoA | comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A412 nm microplate assay | Homo sapiens |