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Literature summary for 2.3.3.10 extracted from

  • Andrew Skaff, D.; Miziorko, H.M.
    A visible wavelength spectrophotometric assay suitable for high-throughput screening of 3-hydroxy-3-methylglutaryl-CoA synthase (2010), Anal. Biochem., 396, 96-102.
    View publication on PubMed

Application

Application Comment Organism
analysis a visible wavelength spectrophotometric assay suitable for high-throughput screening of 3-hydroxy-3-methylglutaryl-CoA synthase is established Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET-HSyn for expression in Escherichia coli cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-benzyl-9-(3,6-dioxocyclohexa-1,4-dienyl)-2,3,7,8-tetrahydro-6H-pyrido[1,2-a]pyrazine-1,4-dione
-
Homo sapiens
3-chloropropionyl-CoA
-
Homo sapiens
3-hydroxy-3-methylglutaryl-CoA
-
Homo sapiens
acetyloxy(phenyl)mercury phenyl mercuric acetate Homo sapiens
diethylcarbamothioylsulfanyl N,N-diethylcarbamodithioate disulfiram Homo sapiens
hymeglusin
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.073
-
acetyl-CoA comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A412 nm standard assay Homo sapiens
0.076
-
acetyl-CoA comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A300 nm standard assay Homo sapiens
0.084
-
acetyl-CoA comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A412 nm microplate assay Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + H2O + acetoacetyl-CoA Homo sapiens
-
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
ir

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + acetoacetyl-CoA
-
Homo sapiens (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
ir

Synonyms

Synonyms Comment Organism
3-hydroxy-3-methylglutaryl-CoA synthase
-
Homo sapiens
HMG-CoA
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24
-
activity assay, A412 nm assay Homo sapiens
30
-
activity assay, A300 nm assay Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
activity assay Homo sapiens

Cofactor

Cofactor Comment Organism Structure
CoA
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0011
-
3-hydroxy-3-methylglutaryl-CoA comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A300 nm standard assay Homo sapiens
0.0015
-
3-hydroxy-3-methylglutaryl-CoA comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A412 nm standard assay Homo sapiens
0.0031
-
3-hydroxy-3-methylglutaryl-CoA comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A412 nm microplate assay Homo sapiens