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Literature summary for 2.3.3.10 extracted from

  • Miziorko, H.M.
    3-Hydroxy-3-methylglutaryl-CoA synthase from chicken liver (1988), Methods Enzymol., 110, 19-26.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
3-carboxy-2,2,5,5,-tetramethyl-1-pyrrolidinyloxyl-CoA
-
Gallus gallus
acetoacetyl-CoA
-
Gallus gallus
DL-3-hydroxy-3-methylglutaryl-CoA competitive inhibition Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00035
-
acetoacetyl-CoA
-
Gallus gallus
0.019
-
S-(3-oxobutyl)-CoA
-
Gallus gallus
0.1
-
acetyl-CoA
-
Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Gallus gallus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
2 * 53000, SDS-PAGE Gallus gallus
105000
-
sedimentation equlibrium Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus
-
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
ir

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
chicken
-

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.85
-
-
Gallus gallus

Storage Stability

Storage Stability Organism
-85┬░C, in presence of 30% glycerol stable for over 1 year Gallus gallus
4┬░C, in presence of 30% glycerol stable for several days Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxobutyryl-CoA + acetoacetyl-CoA + H2O
-
Gallus gallus 3-hydroxy-3-methyl-5-oxoheptanedioyl-CoA + CoA
-
?
acetyl-3'-dephospho-CoA + acetoacetyl-CoA + H2O
-
Gallus gallus 3-hydroxy-3-methylglutaryl-CoA + 3'-dephospho-CoA
-
?
acetyl-CoA + acetoacetyl-CoA + H2O
-
Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
ir
acetyl-CoA + N-acetyl-S-acetoacetylcysteamine + H2O
-
Gallus gallus ? + CoA
-
?
H2O + acetyl-CoA + S-(3-oxobutyl)-CoA
-
Gallus gallus CoA + S-(4-carboxy-3-hydroxy-isoamyl)-CoA
-
r

Subunits

Subunits Comment Organism
dimer 2 * 53000, SDS-PAGE Gallus gallus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
acetoacetyl-CoA
-
Gallus gallus
0.012
-
DL-3-hydroxy-3-methylglutaryl-CoA
-
Gallus gallus