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Literature summary for 2.3.3.10 extracted from
- 3-Hydroxy-3-methylglutaryl-CoA synthase from baker's yeast (1975), Methods Enzymol., 35 B, 173-177.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| enzyme is labile to freezing and thawing, stabilized by glycerol | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetoacetyl-CoA | - |
Saccharomyces cerevisiae |  |
| Cd2+ | - |
Saccharomyces cerevisiae | |
| CoA | - |
Saccharomyces cerevisiae | |
| iodoacetamide | - |
Saccharomyces cerevisiae | |
| iodoacetate | - |
Saccharomyces cerevisiae | |
| N-ethylmaleimide | - |
Saccharomyces cerevisiae | |
| p-chloromercuribenzoate | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
acetoacetyl-CoA | pH 8.9 | Saccharomyces cerevisiae | |
| 0.014 | - |
acetyl-CoA | - |
Saccharomyces cerevisiae | |
| 0.018 | - |
acetyl-CoA | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + acetoacetyl-CoA + H2O | Saccharomyces cerevisiae | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.1 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°, stored in presence of dithiothreitol and 30-50% glycerol purified enzyme retains complete activity for up to 3 months | Saccharomyces cerevisiae |
| 4°C, enzyme loses activity in absence of glycerol | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-3'-dephospho-CoA + acetoacetyl-CoA + H2O | - |
Saccharomyces cerevisiae | 3-hydroxy-3-methylglutaryl-CoA + 3'-dephospho-CoA | - |
? | |
| acetyl-CoA + acetoacetyl-ACP + H2O | - |
Saccharomyces cerevisiae | 3-hydroxy-3-methylglutaryl-ACP | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Saccharomyces cerevisiae | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetylglutathione + acetoacetyl-CoA + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| acetylpantetheine + acetoacetyl-CoA + H2O | - |
Saccharomyces cerevisiae | 3-hydroxy-3-methylglutaryl-CoA + pantetheine | - |
? | |
| additional information | absolutely specific for the acyl-moiety of both its substrates, neither propionyl- nor butyryl CoA can replace acetyl-CoA | Saccharomyces cerevisiae | ? | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
acetoacetyl-CoA | pH 8.9 | Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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