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Literature summary for 2.3.3.10 extracted from

  • Middleton, B.; Tubbs, P.K.
    The purification and some properties of 3-hydroxy-3-methylglutaryl-coenzyme A synthase from baker's yeast (1972), Biochem. J., 126, 27-34.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
enzyme is labile to freezing and thawing, stabilized by glycerol Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) residual activity 51% Saccharomyces cerevisiae
arsenite in presence of CoA, residual activity 25% Saccharomyces cerevisiae
bromoacetyl-CoA
-
Saccharomyces cerevisiae
CdSO4 residual activity 9% Saccharomyces cerevisiae
iodoacetamide residual activity 84% Saccharomyces cerevisiae
additional information desulfo-CoA protects against bromoacetyl-CoA inhibition, but not against p-chloromercuribenzoate and N-ethylmaleimide Saccharomyces cerevisiae
N-ethylmaleimide residual activity 46% Saccharomyces cerevisiae
NaAsO2 residual activity 90% Saccharomyces cerevisiae
p-chloromercuribenzoate residual activity 16% Saccharomyces cerevisiae
phenyl arsenious oxide in presence of CoA, residual activity 41%; residual activity 75% Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O Saccharomyces cerevisiae synthesis of isoprenes and steroids (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
baker's yeast
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O
-
Saccharomyces cerevisiae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?
acetyl-CoA + acetoacetyl-CoA + H2O synthesis of isoprenes and steroids Saccharomyces cerevisiae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?
additional information acetyl-CoA analogues can act as substrates, acetoacyl-analogues are no substrates Saccharomyces cerevisiae ?
-
?