Literature summary for 2.3.2.B2 extracted from

De Pascale, G.; Lloyd, A.J.; Schouten, J.A.; Gilbey, a.M.; Roper, D.I.; Dowson, C.G.; Bugg, T.D.H.
Kinetic characterization of lipid II-Ala:alanyl-tRNA ligase (MurN) from Streptococcus pneumoniae using semisynthetic aminoacyl-lipid II substrates (2008), J. Biol. Chem., 283, 34751-34759.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Streptococcus pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
33.1	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine	pH 6.8, 37°C	Streptococcus pneumoniae
40	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine	pH 6.8, 37°C	Streptococcus pneumoniae
125	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine	pH 6.8, 37°C	Streptococcus pneumoniae
146	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine	pH 6.8, 37°C	Streptococcus pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	-	penicillin-resistant strain	-
Streptococcus pneumoniae	-	penicillin-sensitive strain	-
Streptococcus pneumoniae 159	-	penicillin-resistant strain	-
Streptococcus pneumoniae Pn16	-	penicillin-sensitive strain	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.7	-	substrate UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine, pH 6.8, 37°C	Streptococcus pneumoniae
0.9	-	substrate UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine, pH 6.8, 37°C	Streptococcus pneumoniae
8.7	-	substrate UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine, pH 6.8, 37°C	Streptococcus pneumoniae
11	-	substrate UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine, pH 6.8, 37°C	Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	no substrate: UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine, i.e. lipid I. No addition of L-serine to alanyl-lipid I	Streptococcus pneumoniae	?	-	?
additional information	no substrate: UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine, i.e. lipid I. No addition of L-serine to alanyl-lipid I	Streptococcus pneumoniae 159	?	-	?
additional information	no substrate: UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine, i.e. lipid I. No addition of L-serine to alanyl-lipid I	Streptococcus pneumoniae Pn16	?	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA	-	Streptococcus pneumoniae	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA	i.e. N6-alanyl-lipid I	Streptococcus pneumoniae	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA	-	Streptococcus pneumoniae 159	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA	i.e. N6-alanyl-lipid I	Streptococcus pneumoniae 159	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA	-	Streptococcus pneumoniae Pn16	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA	i.e. N6-alanyl-lipid I	Streptococcus pneumoniae Pn16	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA	-	Streptococcus pneumoniae	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA	i.e. N6-seryl-lipid I	Streptococcus pneumoniae	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA	-	Streptococcus pneumoniae 159	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA	i.e. N6-seryl-lipid I	Streptococcus pneumoniae 159	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA	-	Streptococcus pneumoniae Pn16	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA	i.e. N6-seryl-lipid I	Streptococcus pneumoniae Pn16	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA	-	?

Synonyms

Synonyms	Comment	Organism
lipid II-Ala:alanyl-tRNA ligase	-	Streptococcus pneumoniae
MurN	-	Streptococcus pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.02	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine	pH 6.8, 37°C	Streptococcus pneumoniae
0.06	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine	pH 6.8, 37°C	Streptococcus pneumoniae
0.07	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine	pH 6.8, 37°C	Streptococcus pneumoniae

General Information

General Information	Comment	Organism
physiological function	enzyme is involved in formation of branched peptidoglycan peptide cross-bridges L-Ala-L-Ala or L-Ser-L-Ala in Streptococcus pneumoniae	Streptococcus pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.112	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine	pH 6.8, 37°C	Streptococcus pneumoniae
0.138	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine	pH 6.8, 37°C	Streptococcus pneumoniae
1.579	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine	pH 6.8, 37°C	Streptococcus pneumoniae
2.186	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine	pH 6.8, 37°C	Streptococcus pneumoniae

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Release 2023.1 (January 2023)