Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as Maltose-binding protein fusion | Aspergillus oryzae |
expression in Escherichia coli as Maltose-binding protein fusionand with HA-tag | Yarrowia lipolytica |
expression in Escherichia coli as Maltose-binding protein fusions | Aspergillus fumigatus |
Protein Variants | Comment | Organism |
---|---|---|
K321E/R322E | mutation in the alpha helix, inactive | Aspergillus fumigatus |
K335E | mutation in the alpha helix, inactive | Aspergillus fumigatus |
R217E/R221E | mutations in alpha helix, inactive | Yarrowia lipolytica |
R217E/R221E/R224E/K228E | mutations in alpha helix, inactive | Yarrowia lipolytica |
R224E/K228E | mutations in alpha helix, inactive | Yarrowia lipolytica |
R328E | mutation in the alpha helix, inactive | Aspergillus fumigatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycine + ergosterol | Aspergillus fumigatus | - |
AMP + diphosphate + ergosteryl-3beta-O-glycine | - |
? | |
ATP + glycine + ergosterol | Aspergillus oryzae | - |
AMP + diphosphate + ergosteryl-3beta-O-glycine | - |
? | |
ATP + glycine + ergosterol | Yarrowia lipolytica | - |
AMP + diphosphate + ergosteryl-3beta-O-glycine | - |
? | |
ATP + glycine + ergosterol | Yarrowia lipolytica CLIB 122 | - |
AMP + diphosphate + ergosteryl-3beta-O-glycine | - |
? | |
ATP + glycine + ergosterol | Aspergillus fumigatus ATCC MYA-4609 | - |
AMP + diphosphate + ergosteryl-3beta-O-glycine | - |
? | |
ATP + glycine + ergosterol | Aspergillus oryzae ATCC 42149 | - |
AMP + diphosphate + ergosteryl-3beta-O-glycine | - |
? | |
ATP + glycine + tRNAGly | Aspergillus fumigatus | - |
AMP + diphosphate + L-glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | Aspergillus oryzae | - |
AMP + diphosphate + L-glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | Yarrowia lipolytica | - |
AMP + diphosphate + L-glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | Yarrowia lipolytica CLIB 122 | - |
AMP + diphosphate + L-glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | Aspergillus fumigatus ATCC MYA-4609 | - |
AMP + diphosphate + L-glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | Aspergillus oryzae ATCC 42149 | - |
AMP + diphosphate + L-glycyl-tRNAGly | - |
? | |
ergosterol + L-glycyl-tRNAGly | Aspergillus fumigatus | - |
ergosteryl-3beta-O-glycine + tRNAGly | - |
? | |
ergosterol + L-glycyl-tRNAGly | Aspergillus oryzae | - |
ergosteryl-3beta-O-glycine + tRNAGly | - |
? | |
ergosterol + L-glycyl-tRNAGly | Yarrowia lipolytica | - |
ergosteryl-3beta-O-glycine + tRNAGly | - |
? | |
ergosterol + L-glycyl-tRNAGly | Yarrowia lipolytica CLIB 122 | - |
ergosteryl-3beta-O-glycine + tRNAGly | - |
? | |
ergosterol + L-glycyl-tRNAGly | Aspergillus fumigatus ATCC MYA-4609 | - |
ergosteryl-3beta-O-glycine + tRNAGly | - |
? | |
ergosterol + L-glycyl-tRNAGly | Aspergillus oryzae ATCC 42149 | - |
ergosteryl-3beta-O-glycine + tRNAGly | - |
? | |
additional information | Aspergillus fumigatus | no substrate: glycyl-adenylate | ? | - |
- |
|
additional information | Aspergillus oryzae | no substrate: glycyl-adenylate | ? | - |
- |
|
additional information | Yarrowia lipolytica | no substrate: glycyl-adenylate | ? | - |
- |
|
additional information | Yarrowia lipolytica CLIB 122 | no substrate: glycyl-adenylate | ? | - |
- |
|
additional information | Aspergillus fumigatus ATCC MYA-4609 | no substrate: glycyl-adenylate | ? | - |
- |
|
additional information | Aspergillus oryzae ATCC 42149 | no substrate: glycyl-adenylate | ? | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | Q4WB70 | cf. EC 6.1.1.14 | - |
Aspergillus fumigatus ATCC MYA-4609 | Q4WB70 | cf. EC 6.1.1.14 | - |
Aspergillus oryzae | Q2U0A3 | - |
- |
Aspergillus oryzae ATCC 42149 | Q2U0A3 | - |
- |
Yarrowia lipolytica | Q6C7J3 | cf. EC 6.1.1.14 | - |
Yarrowia lipolytica CLIB 122 | Q6C7J3 | cf. EC 6.1.1.14 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycine + ergosterol | - |
Aspergillus fumigatus | AMP + diphosphate + ergosteryl-3beta-O-glycine | - |
? | |
ATP + glycine + ergosterol | - |
Aspergillus oryzae | AMP + diphosphate + ergosteryl-3beta-O-glycine | - |
? | |
ATP + glycine + ergosterol | - |
Yarrowia lipolytica | AMP + diphosphate + ergosteryl-3beta-O-glycine | - |
? | |
ATP + glycine + ergosterol | - |
Yarrowia lipolytica CLIB 122 | AMP + diphosphate + ergosteryl-3beta-O-glycine | - |
? | |
ATP + glycine + ergosterol | - |
Aspergillus fumigatus ATCC MYA-4609 | AMP + diphosphate + ergosteryl-3beta-O-glycine | - |
? | |
ATP + glycine + ergosterol | - |
Aspergillus oryzae ATCC 42149 | AMP + diphosphate + ergosteryl-3beta-O-glycine | - |
? | |
ATP + glycine + tRNAGly | - |
Aspergillus fumigatus | AMP + diphosphate + L-glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | - |
Aspergillus oryzae | AMP + diphosphate + L-glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | - |
Yarrowia lipolytica | AMP + diphosphate + L-glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | - |
Yarrowia lipolytica CLIB 122 | AMP + diphosphate + L-glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | - |
Aspergillus fumigatus ATCC MYA-4609 | AMP + diphosphate + L-glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | - |
Aspergillus oryzae ATCC 42149 | AMP + diphosphate + L-glycyl-tRNAGly | - |
? | |
ergosterol + L-glycyl-tRNAGly | - |
Aspergillus fumigatus | ergosteryl-3beta-O-glycine + tRNAGly | - |
? | |
ergosterol + L-glycyl-tRNAGly | - |
Aspergillus oryzae | ergosteryl-3beta-O-glycine + tRNAGly | - |
? | |
ergosterol + L-glycyl-tRNAGly | - |
Yarrowia lipolytica | ergosteryl-3beta-O-glycine + tRNAGly | - |
? | |
ergosterol + L-glycyl-tRNAGly | - |
Yarrowia lipolytica CLIB 122 | ergosteryl-3beta-O-glycine + tRNAGly | - |
? | |
ergosterol + L-glycyl-tRNAGly | - |
Aspergillus fumigatus ATCC MYA-4609 | ergosteryl-3beta-O-glycine + tRNAGly | - |
? | |
ergosterol + L-glycyl-tRNAGly | - |
Aspergillus oryzae ATCC 42149 | ergosteryl-3beta-O-glycine + tRNAGly | - |
? | |
additional information | no substrate: glycyl-adenylate | Aspergillus fumigatus | ? | - |
- |
|
additional information | no substrate: glycyl-adenylate | Aspergillus oryzae | ? | - |
- |
|
additional information | no substrate: glycyl-adenylate | Yarrowia lipolytica | ? | - |
- |
|
additional information | no substrate: glycyl-adenylate | Yarrowia lipolytica CLIB 122 | ? | - |
- |
|
additional information | no substrate: glycyl-adenylate | Aspergillus fumigatus ATCC MYA-4609 | ? | - |
- |
|
additional information | no substrate: glycyl-adenylate | Aspergillus oryzae ATCC 42149 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
AFUA_8g01260 | - |
Aspergillus fumigatus |
AO090011000521 | - |
Aspergillus oryzae |
DUF2156 domain-containing protein | - |
Aspergillus fumigatus |
DUF2156 domain-containing protein | - |
Aspergillus oryzae |
DUF2156 domain-containing protein | - |
Yarrowia lipolytica |
ergosteryl-3beta-O-glycine synthase | - |
Aspergillus fumigatus |
ergosteryl-3beta-O-glycine synthase | - |
Aspergillus oryzae |
ergosteryl-3beta-O-glycine synthase | - |
Yarrowia lipolytica |
ErgS | - |
Aspergillus fumigatus |
ErgS | - |
Aspergillus oryzae |
ErgS | - |
Yarrowia lipolytica |
YALI0E00330g | - |
Yarrowia lipolytica |
General Information | Comment | Organism |
---|---|---|
evolution | ErgS consists of a freestanding DUF2156 domain encoded by a gene distinct from and paralogous to that of ergosteryl-3beta-O-L-aspartate synthase ErdS. The enzyme only uses Gly-tRNAGly produced by an independent glycyl-tRNA synthetase (GlyRS) to transfer glycine onto the 3beta-OH of ergosterol, producing ergosteryl-3beta-O-glycine. The ergosteryl-3beta-O-glycine synthesis pathway exists only in Ascomycota | Aspergillus fumigatus |
evolution | ErgS consists of a freestanding DUF2156 domain encoded by a gene distinct from and paralogous to that of ergosteryl-3beta-O-L-aspartate synthase ErdS. The enzyme only uses Gly-tRNAGly produced by an independent glycyl-tRNA synthetase (GlyRS) to transfer glycine onto the 3beta-OH of ergosterol, producing ergosteryl-3beta-O-glycine. The ergosteryl-3beta-O-glycine synthesis pathway exists only in Ascomycota | Aspergillus oryzae |
evolution | ErgS consists of a freestanding DUF2156 domain encoded by a gene distinct from and paralogous to that of ergosteryl-3beta-O-L-aspartate synthase ErdS. The enzyme only uses Gly-tRNAGly produced by an independent glycyl-tRNA synthetase (GlyRS) to transfer glycine onto the 3beta-OH of ergosterol, producing ergosteryl-3beta-O-glycine. The ergosteryl-3beta-O-glycine synthesis pathway exists only in Ascomycota | Yarrowia lipolytica |