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Literature summary for 2.3.2.B14 extracted from

  • Magnet, S.; Arbeloa, A.; Mainardi, J.L.; Hugonnet, J.E.; Fourgeaud, M.; Dubost, L.; Marie, A.; Delfosse, V.; Mayer, C.; Rice, L.B.; Arthur, M.
    Specificity of L,D-transpeptidases from Gram-positive bacteria producing different peptidoglycan chemotypes (2007), J. Biol. Chem., 282, 13151-13159.
    View publication on PubMed
Search for more literature for 2.3.2.B14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Bacillus subtilis
expressed in Escherichia coli Enterococcus faecalis
expressed in Escherichia coli Enterococcus faecium

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis 168
-
-
-
Enterococcus faecalis
-
-
-
Enterococcus faecalis JH2-2
-
-
-
Enterococcus faecium
-
-
-
Enterococcus faecium M512
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity anion exchange chromatography and gel filtration Enterococcus faecium
Ni2+ nitrilotriacetate-agarose resin affinity chromatography Bacillus subtilis
Ni2+ nitrilotriacetate-agarose resin affinity chromatography, MonoQ HR5/5 chromatography, and Superdex HR10/30 column gel filtration Enterococcus faecalis

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris-HCl pH 7.5 containing 100 mM NaCl Bacillus subtilis
-80 °C, 50 mM Tris-HCl pH 7.5 containing 300 mM NaCl Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(L-Lys)3-D-iso-Asn + (L-Lys)3-L-Ala-L-Ala
-
 Enterococcus faecium ?
-
 ?
(L-Lys)3-D-iso-Asn + (L-Lys)3-L-Ala-L-Ala
-
 Enterococcus faecium M512 ?
-
 ?
(L-Lys)3-L-Ala-L-Ala + ?
-
 Bacillus subtilis ?
-
 ?
(L-Lys)3-L-Ala-L-Ala + ?
-
 Bacillus subtilis 168 ?
-
 ?
meso-diaminopimelic acid + (L-Lys)3-D-iso-Asn
-
 Enterococcus faecalis ?
-
 ?
meso-diaminopimelic acid + (L-Lys)3-D-iso-Asn
-
 Enterococcus faecalis JH2-2 ?
-
 ?
meso-diaminopimelic acid + (L-Lys)3-L-Ala-L-Ala
-
 Enterococcus faecalis ?
-
 ?
meso-diaminopimelic acid + (L-Lys)3-L-Ala-L-Ala
-
 Enterococcus faecalis JH2-2 ?
-
 ?
additional information the enzyme is specific for disaccharide-peptides containing an L-Ala-L-Ala side chain in both the acceptor and donor substrates Bacillus subtilis ?
-
 ?
additional information the enzyme is specific for disaccharide-peptides containing an L-Ala-L-Ala side chain in both the acceptor and donor substrates Bacillus subtilis 168 ?
-
 ?

Synonyms

Synonyms Comment Organism
L,D-transpeptidase
-
 Bacillus subtilis
L,D-transpeptidase
-
 Enterococcus faecalis
L,D-transpeptidase
-
 Enterococcus faecium
LdtBS
-
 Bacillus subtilis
Ldtfm
-
 Enterococcus faecium
Ldtfs
-
 Enterococcus faecalis