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Literature summary for 2.3.2.7 extracted from

  • Jayaram, H.N.; Ramakrishnan, T.; Vaidyanathan, C.S.
    Aspartotransferase from Mycobacterium tuberculosis H37Ra (1969), Indian J. Biochem., 6, 106-110.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
Isonicotinic acid hydrazide activation Mycobacterium tuberculosis
L-cystine 20 mM, slight activation Mycobacterium tuberculosis

General Stability

General Stability Organism
dialysis against 0.05 M Tris buffer, pH 7.0, 75% loss of activity Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
Mycobacterium tuberculosis
Cu2+
-
Mycobacterium tuberculosis
L-aspartate 20 mM, 30% inhibition Mycobacterium tuberculosis
L-cysteine 5 mM, 50% inhibition Mycobacterium tuberculosis
additional information no inhibition by NaN3, isonicotinic acid hydrazide, L-homoserine, L-glutamic acid, terminal amino acids of aspartic acid pathway, i.e. lysine, threonine, methionine and isoleucine, up to 20 mM Mycobacterium tuberculosis
PCMB reversible by GSH, 2-mercaptoethanol or 2,3-dimercaptopropanol, not L-cysteine Mycobacterium tuberculosis
streptomycin 5 mM, 50% inhibition Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.14
-
L-asparagine
-
Mycobacterium tuberculosis
19.3
-
hydroxylamine
-
Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ion requirement Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + hydroxylamine Mycobacterium tuberculosis involved in initial step of asparagine metabolism NH3 + beta-L-aspartohydroxamic acid
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
H37Ra, strain number 7417
-

Purification (Commentary)

Purification (Comment) Organism
-
Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0245
-
-
Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
asparagine + hydroxylamine strict specificity, L- and D-enantiomer equally effective, no substrate: L-glutamine Mycobacterium tuberculosis NH3 + beta-aspartohydroxamic acid
-
?
L-asparagine + hydroxylamine involved in initial step of asparagine metabolism Mycobacterium tuberculosis NH3 + beta-L-aspartohydroxamic acid
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Mycobacterium tuberculosis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 40°C: about 50% of maximal activity, 60°C: about 80% of maximal activity Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
6 h, 23% loss of activity Mycobacterium tuberculosis
28
-
6 h, stable Mycobacterium tuberculosis
37
-
6 h, about 8% loss of activity Mycobacterium tuberculosis
60
-
10 min, about 45% loss of activity Mycobacterium tuberculosis
65
-
10 min, about 66% loss of activity Mycobacterium tuberculosis
70
-
t1/2: 1 min, about 55% loss of activity after 2 min Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
8.5 9.4 about half-maximal activity at pH 8.5 and 9.4 Mycobacterium tuberculosis