Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Isonicotinic acid hydrazide | activation | Mycobacterium tuberculosis | |
L-cystine | 20 mM, slight activation | Mycobacterium tuberculosis |
General Stability | Organism |
---|---|
dialysis against 0.05 M Tris buffer, pH 7.0, 75% loss of activity | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Mycobacterium tuberculosis | |
Cu2+ | - |
Mycobacterium tuberculosis | |
L-aspartate | 20 mM, 30% inhibition | Mycobacterium tuberculosis | |
L-cysteine | 5 mM, 50% inhibition | Mycobacterium tuberculosis | |
additional information | no inhibition by NaN3, isonicotinic acid hydrazide, L-homoserine, L-glutamic acid, terminal amino acids of aspartic acid pathway, i.e. lysine, threonine, methionine and isoleucine, up to 20 mM | Mycobacterium tuberculosis | |
PCMB | reversible by GSH, 2-mercaptoethanol or 2,3-dimercaptopropanol, not L-cysteine | Mycobacterium tuberculosis | |
streptomycin | 5 mM, 50% inhibition | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.14 | - |
L-asparagine | - |
Mycobacterium tuberculosis | |
19.3 | - |
hydroxylamine | - |
Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ion requirement | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + hydroxylamine | Mycobacterium tuberculosis | involved in initial step of asparagine metabolism | NH3 + beta-L-aspartohydroxamic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
H37Ra, strain number 7417 | - |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0245 | - |
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
asparagine + hydroxylamine | strict specificity, L- and D-enantiomer equally effective, no substrate: L-glutamine | Mycobacterium tuberculosis | NH3 + beta-aspartohydroxamic acid | - |
? | |
L-asparagine + hydroxylamine | involved in initial step of asparagine metabolism | Mycobacterium tuberculosis | NH3 + beta-L-aspartohydroxamic acid | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Mycobacterium tuberculosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | 40°C: about 50% of maximal activity, 60°C: about 80% of maximal activity | Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
6 h, 23% loss of activity | Mycobacterium tuberculosis |
28 | - |
6 h, stable | Mycobacterium tuberculosis |
37 | - |
6 h, about 8% loss of activity | Mycobacterium tuberculosis |
60 | - |
10 min, about 45% loss of activity | Mycobacterium tuberculosis |
65 | - |
10 min, about 66% loss of activity | Mycobacterium tuberculosis |
70 | - |
t1/2: 1 min, about 55% loss of activity after 2 min | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.4 | about half-maximal activity at pH 8.5 and 9.4 | Mycobacterium tuberculosis |