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Literature summary for 2.3.2.6 extracted from

  • Taki, M.; Kuroiwa, H.
    Unexpectedly fast transfer of positron-emittable artificial substrate into N-terminus of peptide/protein mediated by wild-type L/F-tRNA-protein transferase (2015), Amino Acids, 47, 1279-1282 .
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
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additional information steady-state kinetic analysis of L/F-transferase Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A8P1
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanyl-tRNAPhe + N-terminal L-leucyl-[protein]
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Escherichia coli tRNAPhe + N-terminal L-phenylalanyl-L-leucyl-[protein]
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?
additional information various non-natural amino acids as artificial substrates can be enzymatically introduced only at the basic N-terminus of any kind of acceptor peptides/proteins by using Escherichia coli leucyl/phenylalanyl-tRNA-protein transferase (L/F-transferase) without any engineering of its catalytic pocket. Extension of this L/F-transferase-mediated functionalization of peptides/proteins in combination with aminoacyl-tRNA synthetase (ARS) mutant. Fast enzymatic introduction of a positron emission tomography (PET) probe into acceptor peptides/proteins, it is site-specifically introduced at the basic N-terminus of the acceptors by using L/F-transferase in combination with aminoacyl-tRNA synthetase, namely the NEXT-A/PET reaction. Estimated from kinetic analysis, the transfer efficiency of O-(2-fluoromethyl)-L-tyrosine as an artificial amino acid PET probe mediated by the wild-type transferase is almost as good as that of the natural substrate, phenylalanine. About 90 % of Lys-Ala-7-amino-4-methylcoumarin peptide or of Lys-bradykinin peptide are labeled by O-(2-fluoromethyl)-L-tyrosine (FMT), the model protein Lys-SoCBM13 is also labeled by FMT Escherichia coli ?
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O-(2-fluoromethyl)-L-tyrosinyl-tRNA + N-terminal L-leucyl-[protein]
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Escherichia coli tRNA + N-terminal O-(2-fluoromethyl)-L-tyrosinyl-L-leucyl-[protein]
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?

Synonyms

Synonyms Comment Organism
AAT
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Escherichia coli
L/F-transferase
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Escherichia coli
L/F-tRNA-protein transferase
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Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
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assay at Escherichia coli