BRENDA - Enzyme Database
show all sequences of 2.3.2.6

Kinetic analysis of the leucyl/phenylalanyl-tRNA-protein transferase with acceptor peptides possessing different N-terminal penultimate residues

Kawaguchi, J.; Maejima, K.; Kuroiwa, H.; Taki, M.; FEBS open bio 3, 252-255 (2013)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0012
-
KAC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
0.0051
-
KQC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
0.011
-
RGPCRAFI
at pH 7.6 and 37°C
Escherichia coli
0.021
-
KPC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-phenylalanyl-tRNA + KAC-acrydonylalanine
-
736113
Escherichia coli
tRNA + L-phenylalanyl-KAC-acrydonylalanine
-
-
-
?
L-phenylalanyl-tRNA + KPC-acrydonylalanine
-
736113
Escherichia coli
tRNA + L-phenylalanyl-KPC-acrydonylalanine
-
-
-
?
L-phenylalanyl-tRNA + KQC-acrydonylalanine
-
736113
Escherichia coli
tRNA + L-phenylalanyl-KQC-acrydonylalanine
-
-
-
?
L-phenylalanyl-tRNA + RGPCRAFI
-
736113
Escherichia coli
tRNA + L-phenylalanyl-RGPCRAFI
-
-
-
?
O-(2-fluoroethyl)- L-tyrosyl-tRNA + acceptor protein
-
736113
Escherichia coli
tRNA + O-(2-fluoroethyl)-L-tyrosyl-protein
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
L/F-transferase
-
Escherichia coli
leucyl/phenylalanyl-tRNA-protein transferase
-
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.25
-
KAC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
0.5
-
KQC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
0.8
-
KPC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
1
-
RGPCRAFI
at pH 7.6 and 37°C
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0012
-
KAC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
0.0051
-
KQC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
0.011
-
RGPCRAFI
at pH 7.6 and 37°C
Escherichia coli
0.021
-
KPC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-phenylalanyl-tRNA + KAC-acrydonylalanine
-
736113
Escherichia coli
tRNA + L-phenylalanyl-KAC-acrydonylalanine
-
-
-
?
L-phenylalanyl-tRNA + KPC-acrydonylalanine
-
736113
Escherichia coli
tRNA + L-phenylalanyl-KPC-acrydonylalanine
-
-
-
?
L-phenylalanyl-tRNA + KQC-acrydonylalanine
-
736113
Escherichia coli
tRNA + L-phenylalanyl-KQC-acrydonylalanine
-
-
-
?
L-phenylalanyl-tRNA + RGPCRAFI
-
736113
Escherichia coli
tRNA + L-phenylalanyl-RGPCRAFI
-
-
-
?
O-(2-fluoroethyl)- L-tyrosyl-tRNA + acceptor protein
-
736113
Escherichia coli
tRNA + O-(2-fluoroethyl)-L-tyrosyl-protein
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.25
-
KAC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
0.5
-
KQC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
0.8
-
KPC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
1
-
RGPCRAFI
at pH 7.6 and 37°C
Escherichia coli
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
40
-
KPC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
96.7
-
KQC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
216.7
-
KAC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
916.7
-
RGPCRAFI
at pH 7.6 and 37°C
Escherichia coli
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
40
-
KPC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
96.7
-
KQC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
216.7
-
KAC-acrydonylalanine
at pH 7.6 and 37°C
Escherichia coli
916.7
-
RGPCRAFI
at pH 7.6 and 37°C
Escherichia coli
Other publictions for EC 2.3.2.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
758629
Taki
Unexpectedly fast transfer of ...
Escherichia coli
Amino Acids
47
1279-1282
2015
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
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3
-
3
1
-
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1
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-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
759102
Fung
The molecular basis for the p ...
Escherichia coli
Curr. Protein Pept. Sci.
16
163-180
2015
-
-
-
-
-
-
-
-
-
-
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3
-
6
-
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6
-
6
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3
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6
-
-
-
-
-
-
-
-
-
-
4
4
-
-
-
737201
Fung
Probing the leucyl/phenylalany ...
Escherichia coli
Protein Pept. Lett.
21
603-614
2014
-
-
-
1
-
-
1
-
-
-
-
2
-
1
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2
-
2
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1
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1
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2
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2
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-
-
-
737230
Fung
The determination of tRNALeu r ...
Escherichia coli
RNA
20
1210-1222
2014
-
-
-
-
-
-
-
2
-
-
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-
-
6
-
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3
-
2
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2
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2
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3
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-
2
-
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-
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-
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-
736113
Kawaguchi
Kinetic analysis of the leucyl ...
Escherichia coli
FEBS open bio
3
252-255
2013
-
-
-
-
-
-
-
4
-
-
-
-
-
1
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5
-
2
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4
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4
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5
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-
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-
4
-
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-
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-
-
4
4
720253
Fung
An alternative mechanism for t ...
Escherichia coli
J. Mol. Biol.
409
617-629
2011
-
-
1
-
7
-
-
-
-
-
-
2
-
4
-
-
1
-
-
-
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-
3
-
2
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-
1
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-
7
-
-
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-
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2
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-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701685
Ebhardt
Quantification of the post-tra ...
Escherichia coli
Anal. Chem.
81
1937-1943
2009
-
1
1
-
-
-
-
4
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
-
3
1
-
-
4
1
-
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-
1
1
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-
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-
4
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2
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-
-
4
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
703447
Ninnis
Modification of PATase by L/F- ...
Escherichia coli
EMBO J.
28
1732-1744
2009
-
-
-
-
-
-
-
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8
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2
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3
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2
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-
-
-
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-
1
1
-
-
-
677035
Dong
The crystal structure of leucy ...
Escherichia coli
Protein Sci.
16
528-534
2007
-
-
1
2
-
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-
-
-
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-
7
-
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2
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1
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-
-
685628
Taki
Leucyl/phenylalanyl(L/F)-tRNA- ...
Escherichia coli
Biopolymers
88
263-271
2007
-
1
-
-
-
-
-
-
-
-
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1
-
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1
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-
689152
Watanabe
Protein-based peptide-bond for ...
Escherichia coli
Nature
449
867-871
2007
-
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-
1
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2
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1
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2
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1
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673077
Taki
Leucyl/phenylalanyl-tRNA-prote ...
Escherichia coli
ChemBiochem
7
1676-1679
2006
-
1
-
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-
-
-
-
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1
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5
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1
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-
5
-
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-
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-
673329
Suto
Crystal structures of leucyl/p ...
Escherichia coli
EMBO J.
25
5942-5950
2006
-
-
-
1
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4
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1
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-
488008
Ichetovkin
Substrate recognition by the l ...
Escherichia coli
J. Biol. Chem.
272
33009-33014
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
22
-
-
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4
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-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488007
Abramochkin
Aminoacyl-tRNA recognition by ...
Escherichia coli
J. Biol. Chem.
271
22901-22907
1996
-
-
-
-
-
-
1
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1
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4
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1
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4
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-
488006
Abrahamochkin
The leucyl/phenylalanyl-tRNA-p ...
Escherichia coli
J. Biol. Chem.
270
20621-20628
1995
-
-
1
-
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5
-
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1
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4
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1
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1
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4
-
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-
-
-
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-
488004
Deutch
Aminoacyl-tRNA: protein transf ...
Escherichia coli
Methods Enzymol.
106
198-205
1984
-
-
-
-
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1
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1
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1
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1
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2
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1
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1
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1
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2
-
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488005
Scarpulla
Transfer of methionyl residues ...
Escherichia coli, Escherichia coli B / ATCC 11303
Biochem. Biophys. Res. Commun.
71
584-589
1976
-
-
-
-
-
-
-
-
1
-
-
-
-
178
-
-
1
-
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1
1
8
-
-
-
-
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1
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1
-
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1
1
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488003
Soffer
Peptide acceptors in the leuci ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Biol. Chem.
248
8424-8428
1973
-
-
-
-
-
1
23
-
1
-
-
-
-
177
-
-
1
-
-
-
-
1
10
-
-
-
-
-
-
-
-
-
-
22
-
-
-
-
-
-
-
-
1
-
23
22
-
1
-
-
-
-
-
-
1
-
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-
1
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488002
Leibowitz
Enzymatic modification of prot ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Biol. Chem.
245
2066-2073
1970
-
-
-
-
-
-
4
-
1
2
-
-
-
177
-
-
1
-
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-
1
1
6
-
-
-
-
1
-
2
1
-
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-
-
-
-
-
4
-
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1
2
-
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-
1
-
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1
1
6
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
488001
Leibowitz
A soluble enzyme from Escheric ...
Escherichia coli, Escherichia coli B / ATCC 11303
Biochem. Biophys. Res. Commun.
36
47-53
1969
-
-
-
-
-
-
-
-
1
3
-
-
-
177
-
-
1
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
1
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655956
Momose
Soluble amino acid-incorporati ...
Escherichia coli
J. Biol. Chem.
241
3294-3207
1966
1
-
-
-
-
-
2
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655954
Kaji
Soluble amino acid-incorporati ...
Escherichia coli
J. Biol. Chem.
240
1185-1191
1965
1
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655955
Kaji
Soluble amino acid-incorporati ...
Escherichia coli
J. Biol. Chem.
240
1192-1197
1965
-
-
-
-
-
-
-
-
1
-
-
-
-
1
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-
-
-
-
-
-
-
1
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-
-
-
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-
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-
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-
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1
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-
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-
-
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1
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