BRENDA - Enzyme Database
show all sequences of 2.3.2.6

Transfer of methionyl residues by leucyl, phenylalanyl-tRNA-protein transferase

Scarpulla, R.C.; Deutch, C.E.; Soffer, R.L.; Biochem. Biophys. Res. Commun. 71, 584-589 (1976)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Escherichia coli
-
-
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
B
-
Escherichia coli
-
K12 strain W4977 and revertant strain R18 of mutant strain MS845
-
Escherichia coli B / ATCC 11303
-
B
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Escherichia coli
Storage Stability
Storage Stability
Organism
-20°C, stable for several months
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-leucyl-tRNALeu + protein
basic NH2-terminal is absolute determinant of specificity
488005
Escherichia coli
tRNALeu + L-leucyl-protein
-
488005
Escherichia coli
?
L-leucyl-tRNALeu + protein
dipeptide specificity
488005
Escherichia coli
tRNALeu + L-leucyl-protein
-
488005
Escherichia coli
?
L-leucyl-tRNALeu + protein
basic NH2-terminal is absolute determinant of specificity
488005
Escherichia coli B / ATCC 11303
tRNALeu + L-leucyl-protein
-
488005
Escherichia coli B / ATCC 11303
?
L-leucyl-tRNALeu + protein
dipeptide specificity
488005
Escherichia coli B / ATCC 11303
tRNALeu + L-leucyl-protein
-
488005
Escherichia coli B / ATCC 11303
?
L-methionyl-tRNA + acceptor protein
methionyl-tRNAmMet preferred to methionyl-tRNAfMet. Peptides containing a basic amino acid at the NH2-terminus function as acceptors
488005
Escherichia coli
tRNA + L-methionyl-protein
-
-
-
?
L-methionyl-tRNA + acceptor protein
methionyl-tRNAmMet preferred to methionyl-tRNAfMet. Peptides containing a basic amino acid at the NH2-terminus function as acceptors
488005
Escherichia coli B / ATCC 11303
tRNA + L-methionyl-protein
-
-
-
?
L-phenylalanyl-tRNA + acceptor protein
-
488005
Escherichia coli
tRNA + L-phenylalanyl-protein
-
-
-
?
L-phenylalanyl-tRNA + acceptor protein
-
488005
Escherichia coli B / ATCC 11303
tRNA + L-phenylalanyl-protein
-
-
-
?
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Escherichia coli
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable for several months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-leucyl-tRNALeu + protein
basic NH2-terminal is absolute determinant of specificity
488005
Escherichia coli
tRNALeu + L-leucyl-protein
-
488005
Escherichia coli
?
L-leucyl-tRNALeu + protein
dipeptide specificity
488005
Escherichia coli
tRNALeu + L-leucyl-protein
-
488005
Escherichia coli
?
L-leucyl-tRNALeu + protein
basic NH2-terminal is absolute determinant of specificity
488005
Escherichia coli B / ATCC 11303
tRNALeu + L-leucyl-protein
-
488005
Escherichia coli B / ATCC 11303
?
L-leucyl-tRNALeu + protein
dipeptide specificity
488005
Escherichia coli B / ATCC 11303
tRNALeu + L-leucyl-protein
-
488005
Escherichia coli B / ATCC 11303
?
L-methionyl-tRNA + acceptor protein
methionyl-tRNAmMet preferred to methionyl-tRNAfMet. Peptides containing a basic amino acid at the NH2-terminus function as acceptors
488005
Escherichia coli
tRNA + L-methionyl-protein
-
-
-
?
L-methionyl-tRNA + acceptor protein
methionyl-tRNAmMet preferred to methionyl-tRNAfMet. Peptides containing a basic amino acid at the NH2-terminus function as acceptors
488005
Escherichia coli B / ATCC 11303
tRNA + L-methionyl-protein
-
-
-
?
L-phenylalanyl-tRNA + acceptor protein
-
488005
Escherichia coli
tRNA + L-phenylalanyl-protein
-
-
-
?
L-phenylalanyl-tRNA + acceptor protein
-
488005
Escherichia coli B / ATCC 11303
tRNA + L-phenylalanyl-protein
-
-
-
?
Other publictions for EC 2.3.2.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
758629
Taki
Unexpectedly fast transfer of ...
Escherichia coli
Amino Acids
47
1279-1282
2015
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1
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1
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3
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1
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1
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3
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1
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759102
Fung
The molecular basis for the p ...
Escherichia coli
Curr. Protein Pept. Sci.
16
163-180
2015
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3
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6
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6
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6
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3
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6
-
-
-
-
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4
4
-
-
-
737201
Fung
Probing the leucyl/phenylalany ...
Escherichia coli
Protein Pept. Lett.
21
603-614
2014
-
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-
1
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1
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2
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1
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2
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737230
Fung
The determination of tRNALeu r ...
Escherichia coli
RNA
20
1210-1222
2014
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2
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6
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3
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2
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2
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2
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3
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2
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-
736113
Kawaguchi
Kinetic analysis of the leucyl ...
Escherichia coli
FEBS open bio
3
252-255
2013
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-
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4
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1
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5
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2
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4
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4
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5
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4
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4
4
720253
Fung
An alternative mechanism for t ...
Escherichia coli
J. Mol. Biol.
409
617-629
2011
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1
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7
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2
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4
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1
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3
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2
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1
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7
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2
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1
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3
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-
701685
Ebhardt
Quantification of the post-tra ...
Escherichia coli
Anal. Chem.
81
1937-1943
2009
-
1
1
-
-
-
-
4
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
-
3
1
-
-
4
1
-
-
-
-
-
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-
1
1
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-
-
-
-
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-
4
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-
-
2
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-
-
-
-
-
4
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
703447
Ninnis
Modification of PATase by L/F- ...
Escherichia coli
EMBO J.
28
1732-1744
2009
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-
-
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8
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2
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3
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2
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1
1
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-
-
677035
Dong
The crystal structure of leucy ...
Escherichia coli
Protein Sci.
16
528-534
2007
-
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1
2
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7
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2
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-
685628
Taki
Leucyl/phenylalanyl(L/F)-tRNA- ...
Escherichia coli
Biopolymers
88
263-271
2007
-
1
-
-
-
-
-
-
-
-
-
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-
1
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1
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-
689152
Watanabe
Protein-based peptide-bond for ...
Escherichia coli
Nature
449
867-871
2007
-
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1
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-
-
-
-
-
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2
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1
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2
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1
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-
673077
Taki
Leucyl/phenylalanyl-tRNA-prote ...
Escherichia coli
ChemBiochem
7
1676-1679
2006
-
1
-
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-
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1
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5
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1
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5
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-
673329
Suto
Crystal structures of leucyl/p ...
Escherichia coli
EMBO J.
25
5942-5950
2006
-
-
-
1
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4
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1
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-
488008
Ichetovkin
Substrate recognition by the l ...
Escherichia coli
J. Biol. Chem.
272
33009-33014
1997
-
-
-
-
-
-
-
-
-
-
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-
22
-
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4
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4
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488007
Abramochkin
Aminoacyl-tRNA recognition by ...
Escherichia coli
J. Biol. Chem.
271
22901-22907
1996
-
-
-
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-
-
1
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1
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4
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-
4
-
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488006
Abrahamochkin
The leucyl/phenylalanyl-tRNA-p ...
Escherichia coli
J. Biol. Chem.
270
20621-20628
1995
-
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1
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5
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1
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1
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4
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488004
Deutch
Aminoacyl-tRNA: protein transf ...
Escherichia coli
Methods Enzymol.
106
198-205
1984
-
-
-
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1
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1
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1
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1
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1
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1
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2
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488005
Scarpulla
Transfer of methionyl residues ...
Escherichia coli, Escherichia coli B / ATCC 11303
Biochem. Biophys. Res. Commun.
71
584-589
1976
-
-
-
-
-
-
-
-
1
-
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-
-
178
-
-
1
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1
1
8
-
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1
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1
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1
1
8
-
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488003
Soffer
Peptide acceptors in the leuci ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Biol. Chem.
248
8424-8428
1973
-
-
-
-
-
1
23
-
1
-
-
-
-
177
-
-
1
-
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1
10
-
-
-
-
-
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22
-
-
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-
1
-
23
22
-
1
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-
1
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1
10
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
488002
Leibowitz
Enzymatic modification of prot ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Biol. Chem.
245
2066-2073
1970
-
-
-
-
-
-
4
-
1
2
-
-
-
177
-
-
1
-
-
-
1
1
6
-
-
-
-
1
-
2
1
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-
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-
4
-
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1
2
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1
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1
1
6
-
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1
-
2
1
-
-
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-
-
-
-
488001
Leibowitz
A soluble enzyme from Escheric ...
Escherichia coli, Escherichia coli B / ATCC 11303
Biochem. Biophys. Res. Commun.
36
47-53
1969
-
-
-
-
-
-
-
-
1
3
-
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-
177
-
-
1
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1
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4
-
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1
3
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1
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1
-
4
-
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655956
Momose
Soluble amino acid-incorporati ...
Escherichia coli
J. Biol. Chem.
241
3294-3207
1966
1
-
-
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2
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1
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1
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2
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655954
Kaji
Soluble amino acid-incorporati ...
Escherichia coli
J. Biol. Chem.
240
1185-1191
1965
1
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2
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3
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655955
Kaji
Soluble amino acid-incorporati ...
Escherichia coli
J. Biol. Chem.
240
1192-1197
1965
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