Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.5 extracted from

  • Wu, Z.; Weng, L.; Zhang, T.; Tian, H.; Fang, L.; Teng, H.; Zhang, W.; Gao, J.; Hao, Y.; Li, Y.; Zhou, H.; Wang, P.
    Identification of glutaminyl cyclase isoenzyme isoQC as a regulator of SIRPalpha-CD47 axis (2019), Cell Res., 29, 502-505 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.5

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and mutant isoQC in isoQC-deficient HEK-293T cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
E225G site-directed mutagenesis, the mutant shows reduced enzymatic activity compared to wild-type Homo sapiens
additional information knockout of isoQC dramatically reduces the binding of SIRPalpha to cell surface Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
PQ529 can efficiently inhibit the activity of both glutaminyl cyclase isozymes QC and isoQC. Treatment with PQ529 for 48 h significantly blocks the binding of CC2C6 to the cell surface in a dosage-dependent manner in wild-type, but not in isoQC-deficient cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Homo sapiens 9986
-
Golgi apparatus
-
 Homo sapiens 5794
-
Golgi membrane unlike QC, the secreted isozyme, isozyme isoQC exclusively localizes within the Golgi complex via a transmembrane domain Homo sapiens 139
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutaminyl-CD47 Homo sapiens
-
 5-oxoprolyl-CD47 + NH3
-
 ?
L-glutaminyl-peptide Homo sapiens
-
 5-oxoprolyl-peptide + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NXS2
-
-

Source Tissue

Source Tissue Comment Organism Textmining
ARP-1 cell
-
 Homo sapiens
-
carcinoma cell
-
 Homo sapiens
-
DLD-1 cell
-
 Homo sapiens
-
HCT-116 cell
-
 Homo sapiens
-
JURKAT cell
-
 Homo sapiens
-
additional information no activity in HEK-293T cells Homo sapiens
-
NCI-H929 cell
-
 Homo sapiens
-
NU-DUL-1 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutaminyl-CD47
-
 Homo sapiens 5-oxoprolyl-CD47 + NH3
-
 ?
L-glutaminyl-peptide
-
 Homo sapiens 5-oxoprolyl-peptide + NH3
-
 ?

Synonyms

Synonyms Comment Organism
glutaminyl cyclase
-
 Homo sapiens
glutaminyl-peptide cyclotransferase-like protein
-
 Homo sapiens
Golgi-resident enzyme
-
 Homo sapiens
isoQC
-
 Homo sapiens
QPCTL
-
 Homo sapiens

General Information

General Information Comment Organism
evolution glutaminyl cyclase (QC, glutaminyl-peptide cyclotransferase (QPCT)) and its isoenzyme isoQC (QPCTL) belong to a family of enzymes which catalyze the formation of pyroglutamate (pGlu, pE) at N-terminus of proteins by converting glutamate/glutamine to pGlu residue Homo sapiens
malfunction knockout of isoQC dramatically reduces the binding of SIRPalpha to cell surface Homo sapiens
metabolism transmembrane protein CD47 is highly expressed on many types of cancer cells and can directly bind to the receptor signal regulatory protein alpha (SIRPalpha), which is highly expressed on phagocytic cells. Binding of CD47 to SIRPalpha can protect cancer cells from phagocytosis by phagocytic cells and therefore functions as the major 'don't eat me' signal. Therapeutic blockade of CD47-SIRPalpha axis can efficiently promote the macrophage-mediated phagocytosis and elimination of cancer cells. Glutaminyl cyclase isoenzyme isoQC is a regulator of SIRPalpha-CD47 axis Homo sapiens
physiological function glutaminyl cyclase isoenzyme isoQC is an essential regulator of CD47-SIRPalpha axis and required for efficient phagocytic cells-mediated clearance of cancer cells. N-terminal pGlu modification of proteins may protect protein from degradation by proteases or promote protein aggregation Homo sapiens