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Literature summary for 2.3.2.5 extracted from

  • Dahl, S.W.; Slaughter, C.; Lauritzen, C.; Bateman, R.C., Jr.; Connerton, I.; Pedersen, J.
    Carica papaya glutamine cyclotransferase belongs to a novel plant enzyme subfamily: cloning and characterization of the recombinant Enzyme (2000), Protein Expr. Purif., 20, 27-36.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as either His-tagged enzyme with three different signal peptides and in fusions with three different signal peptides and in fusion with thioredoxin, glutathione S-transferase, and (pre-)maltose-binding protein. In all cases, the expressed protein is either undetectable or insoluble. Expression in Pichia pastoris of the enzyme fused to the alpha-factor leader results in low levels of activity. Extracellular expression of the enzyme in the insect cell/baculovirus system is sucessfull. Enzyme N-terminally fused to a combined secretion signal/His-tag peptide is correctly processed by the host signal peptidase and the His-tag can subsequently be removed with dipeptidyl peptidase I Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya O81226
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