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Literature summary for 2.3.2.33 extracted from

  • Pao, K.C.; Wood, N.T.; Knebel, A.; Rafie, K.; Stanley, M.; Mabbitt, P.D.; Sundaramoorthy, R.; Hofmann, K.; van Aalten, D.M.F.; Virdee, S.
    Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity (2018), Nature, 556, 381-385 .
    View publication on PubMed
Search for more literature for 2.3.2.33

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
to 1.75 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
C4520 mutation impairs thioester-linked E3-Ub intermediate formation Homo sapiens
C4520A/C4572S loss of activity Homo sapiens
C4572S mutant retains activity and is able to form a discrete mono-ubiquitin adduct Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ a globular domain (residues 4501-4638) binds four Zn ions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens MYCBP2 contains two essential catalytic cysteine residues that relay ubiquitin to its substrate via thioester intermediates. It shows esterification activity and intrinsic selectivity for threonine over serine ?
-
 ?
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-threonine Homo sapiens
-
 [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine
-
 ?
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RCR-type E3 ubiquitin transferase]-L-cysteine Homo sapiens
-
 [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RCR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
-
 ?
[RCR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-threonine Homo sapiens
-
 [RCR-type E3 ubiquitin transferase]-L-cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O75592
-
-

Source Tissue

Source Tissue Comment Organism Textmining
neuron neuron-associated Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information MYCBP2 contains two essential catalytic cysteine residues that relay ubiquitin to its substrate via thioester intermediates. It shows esterification activity and intrinsic selectivity for threonine over serine Homo sapiens ?
-
 ?
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-threonine
-
 Homo sapiens [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine
-
 ?
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RCR-type E3 ubiquitin transferase]-L-cysteine
-
 Homo sapiens [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RCR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
-
 ?
[RCR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-threonine
-
 Homo sapiens [RCR-type E3 ubiquitin transferase]-L-cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine
-
 ?

Synonyms

Synonyms Comment Organism
E3 ubiquitin-protein ligase
-
 Homo sapiens
MYCBP2
-
 Homo sapiens
PHR1
-
 Homo sapiens