Literature summary for 2.3.2.32 extracted from

Kurz, T.; Chou, Y.; Willems, A.; Meyer-Schaller, N.; Hecht, M.; Tyers, M.; Peter, M.; Sicheri, F.
Dcn1 functions as a scaffold-type E3 ligase for cullin neddylation (2008), Mol. Cell., 29, 23-35 .

Search for more literature for 2.3.2.32

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 400 mM ammonium acetate, 30% (w/v) PEG4000, and 0.1 M trisodium citrate (pH 5.6) at 20°C	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
A253R	inactive	Saccharomyces cerevisiae
A253R/D259A	inactive	Saccharomyces cerevisiae
D226A	inactive	Saccharomyces cerevisiae
D226A/A253R	inactive	Saccharomyces cerevisiae
D226A/A253R/D259A	inactive	Saccharomyces cerevisiae
D226A/D259A	inactive	Saccharomyces cerevisiae
D259A	inactive	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
[E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine + [cullin Cdc53]-L-lysine	Saccharomyces cerevisiae	-	[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin Cdc53]-N6-[NEDD8-protein]-yl-L-lysine	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	Q12395	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap column chromatography	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine + [cullin Cdc53]-L-lysine	-	Saccharomyces cerevisiae	[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin Cdc53]-N6-[NEDD8-protein]-yl-L-lysine	-	?

Synonyms

Synonyms	Comment	Organism
Dcn1	-	Saccharomyces cerevisiae
Nedd8 E3 ligase	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)