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Literature summary for 2.3.2.3 extracted from

  • Roy, H.; Ibba, M.
    Monitoring Lys-tRNA(Lys) phosphatidylglycerol transferase activity (2008), Methods, 44, 164-169.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis a method to obtain a stable enriched membrane fraction containing MprF, and the techniques necessary to quantitatively monitor its activity in vitro and in vivo is reported Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET33b for expression in Escherichia coli Rosetta2 DE3, C41 and C43 cells Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Bacillus subtilis 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
determined by SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysyl-tRNALys + phosphatidylglycerol Bacillus subtilis
-
tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
membrane fractions are prepared by differential centrifugation Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00000072
-
supernatant 10000 x g Bacillus subtilis
0.00000143
-
cell crude extract Bacillus subtilis
0.00000326
-
membrane fraction Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysyl-tRNALys + phosphatidylglycerol
-
Bacillus subtilis tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol
-
?

Synonyms

Synonyms Comment Organism
Lys-tRNA(Lys) phosphatidylglycerol transferase
-
Bacillus subtilis
MprF
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
steady state assay Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
steady state assay Bacillus subtilis