Application | Comment | Organism |
---|---|---|
analysis | a method to obtain a stable enriched membrane fraction containing MprF, and the techniques necessary to quantitatively monitor its activity in vitro and in vivo is reported | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
into the vector pET33b for expression in Escherichia coli Rosetta2 DE3, C41 and C43 cells | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Bacillus subtilis | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
determined by SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysyl-tRNALys + phosphatidylglycerol | Bacillus subtilis | - |
tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
membrane fractions are prepared by differential centrifugation | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00000072 | - |
supernatant 10000 x g | Bacillus subtilis |
0.00000143 | - |
cell crude extract | Bacillus subtilis |
0.00000326 | - |
membrane fraction | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysyl-tRNALys + phosphatidylglycerol | - |
Bacillus subtilis | tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Lys-tRNA(Lys) phosphatidylglycerol transferase | - |
Bacillus subtilis |
MprF | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
steady state assay | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
steady state assay | Bacillus subtilis |