Crystallization (Comment) | Organism |
---|---|
structure of dimeric Ring finger domain of MEX3C and comparison with the complex structure of MDM2/MDMX-UbcH5b-Ub. The Ring finger domain of MEX3C acts as a ubiquitin E3 ligase in vitro, cooperating with E2 enzymes UbcH5b and UbcH5c to mediate ubiquitination | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C608S/C611S | mutation in Zn2+ coordination site, almost complete loss ofactivity | Homo sapiens |
C632S | mutation in Zn2+ coordination site, almost complete loss of activity | Homo sapiens |
I610A | mutation abolishes selfubiquitination activity. Residue may play key role to interact with E2 and stabilize ubiquitin in the closed active E2-Ub conformation | Homo sapiens |
I636A | mutation abolishes selfubiquitination activity | Homo sapiens |
K639A | mutation reduces selfubiquitination activity | Homo sapiens |
P645A | mutation abolishes selfubiquitination activity | Homo sapiens |
Q648A | mutation abolishes selfubiquitination activity. Residue may play key role to interact with E2 and stabilize ubiquitin in the closed active E2-Ub conformation | Homo sapiens |
V609A | mutation reduces selfubiquitination activity | Homo sapiens |
V609E | mutation abolishes selfubiquitination activity | Homo sapiens |
V646E | mutation abolishes selfubiquitination activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTPA | treatment leads to inactivation | Homo sapiens | |
EDTA | treatment leads to inactivation | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | absolutely required | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q5U5Q3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[E2 ubiquitin-conjugating enzyme UbcH5b]-S-ubiquitinyl-L-cysteine + [Ring finger domain of MEX3C]-L-lysine | - |
Homo sapiens | [E2 ubiquitin-conjugating enzyme UbcH5b]-L-cysteine + [Ring finger domain of MEX3C]-N6-ubiquitinyl-L-lysine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MEX3C | - |
Homo sapiens |