Crystallization (Comment) | Organism |
---|---|
crystal structure of ZNRF1 C-terminal domain in complex with Ube2N. The domain binds Ube2N exclusively via its RING domain. The ZNRF1:Ube2N interface contains three salt-bridges/H-bonds involving Glu183 of ZNRF1 and Arg14/Lys10 of Ube2N | Homo sapiens |
Organism | UniProt | Comment | Textmining |
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Homo sapiens | Q8ND25 | - |
- |
Synonyms | Comment | Organism |
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ZNRF1 | - |
Homo sapiens |
General Information | Comment | Organism |
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physiological function | the RING domain of E3 ligase ZNRF1 binds Ube2N with a Kd of about 50 nM. ZNRF1 interacts with Ube2D2 with a Kd of about 1 mM. The ubiquitination efficiency of ZNRF1:E2 pairs correlates with their affinity. An excess of ZNRF1 inhibits Ube2N-mediated ubiquitination at concentrations at or above 500 nM instead of showing enhanced ubiquitination | Homo sapiens |