Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ARRDC1 | alpha-arrestin, anchoring an ARRDC1-GFP construct to the membrane results in efficient activation and autoubiquitination of NEDD4 ligase | Homo sapiens | |
Ndfip2 | adaptor protein containing multiple PY domains | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00308 | isoform WWP2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [Dvl2]-L-lysine | - |
Homo sapiens | [E2 ubiquitin-conjugating enzyme]-L-cysteine + [Dvl2]-N6-ubiquitinyl-L-lysine | - |
? |
General Information | Comment | Organism |
---|---|---|
physiological function | NEDD4 ligase activation critically requires a substantial array of clustered PY motifs. Soluble protein substrates and adaptors such as alpha-arrestins, even with multiple PY elements, cannot activate ligase activity efficiently. Polymerization or membrane tethering of these substrates dramatically increases the ligase activity both in vivo and in vitro. Aggregation of luciferase-containing substrates upon heat shock has a similar effect and can also expose cryptic PY elements in the substrates | Homo sapiens |