Literature summary for 2.3.2.25 extracted from

Scaglione, K.M.; Basrur, V.; Ashraf, N.S.; Konen, J.R.; Elenitoba-Johnson, K.S.; Todi, S.V.; Paulson, H.L.
The ubiquitin-conjugating enzyme (E2) Ube2w ubiquitinates the N terminus of substrates (2013), J. Biol. Chem., 288, 18784-18788.

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Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid = [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[acceptor protein]	in contrast to other ubiquitin-conjugating enzymes which form an isopeptide bond, this enzyme catalyzes the formation of a peptide bond at the N-terminus of the acceptor protein	Homo sapiens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	Ube2w is not reactive toward free lysine and contains novel residues in its active site that are important for activity	Homo sapiens	?	to confirm N-terminal ubiquitination lysine-less and N-terminally blocked substrates are generated. The lysine-less substrate is ubiquinated, but not the N-terminally blocked one	?
[ubiquitin-carrier protein E2]-S-ubiquitinyl-L-cysteine + [ataxin-3]-NH2	-	Homo sapiens	[ubiquitin-carrier protein E2]-L-cysteine + N-terminal-ubiquitinyl-[ataxin-3]	-	?
[ubiquitin-carrier protein E2]-S-ubiquitinyl-L-cysteine + [protein-tau]-NH2	-	Homo sapiens	[ubiquitin-carrier protein E2]-L-cysteine + N-terminal-ubiquitinyl-[protein-tau]	-	?

Synonyms

Synonyms	Comment	Organism
E2 Ube2w	-	Homo sapiens
E2 ubiquitin-conjugating enzyme	-	Homo sapiens
UBE2W	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)