Cloned (Comment) | Organism |
---|---|
gene UBE2K, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D127G | site-directed mutagenesis | Homo sapiens |
additional information | molecular dynamics simulations for E2-25K and the Q126L/D127G active site gate mutants | Homo sapiens |
Q126A | site-directed mutagenesis | Homo sapiens |
Q126G | site-directed mutagenesis | Homo sapiens |
Q126I | site-directed mutagenesis | Homo sapiens |
Q126L | site-directed mutagenesis | Homo sapiens |
Q126V | site-directed mutagenesis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine | Homo sapiens | - |
[E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P61086 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, followed by tag cleavage by TEV protease, dialysis, and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | determination of the E1 catalyzed E2-25K-Ub thioester conjugation reaction | Homo sapiens | ? | - |
- |
|
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine | - |
Homo sapiens | [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
E2-25K | - |
Homo sapiens |
ubiquitination enzyme | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | active site gate dynamics modulate the catalytic activity of the ubiquitination enzyme E2-25K. NMR study of purified recombinant detagged wild-type and mutant enzymes, analysis of enzyme structure and E2-25K-ubiquitin interaction, overview. Molecular dynamics simulations for E2-25K and the Q126L/D127G active site gate mutants | Homo sapiens |