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Literature summary for 2.3.2.23 extracted from

  • Rout, M.K.; Lee, B.L.; Lin, A.; Xiao, W.; Spyracopoulos, L.
    Active site gate dynamics modulate the catalytic activity of the ubiquitination enzyme E2-25K (2018), Sci. Rep., 8, 7002 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene UBE2K, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
D127G site-directed mutagenesis Homo sapiens
additional information molecular dynamics simulations for E2-25K and the Q126L/D127G active site gate mutants Homo sapiens
Q126A site-directed mutagenesis Homo sapiens
Q126G site-directed mutagenesis Homo sapiens
Q126I site-directed mutagenesis Homo sapiens
Q126L site-directed mutagenesis Homo sapiens
Q126V site-directed mutagenesis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine Homo sapiens
-
[E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P61086
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, followed by tag cleavage by TEV protease, dialysis, and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information determination of the E1 catalyzed E2-25K-Ub thioester conjugation reaction Homo sapiens ?
-
-
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine
-
Homo sapiens [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
-
?

Synonyms

Synonyms Comment Organism
E2-25K
-
Homo sapiens
ubiquitination enzyme
-
Homo sapiens

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
20
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

General Information

General Information Comment Organism
additional information active site gate dynamics modulate the catalytic activity of the ubiquitination enzyme E2-25K. NMR study of purified recombinant detagged wild-type and mutant enzymes, analysis of enzyme structure and E2-25K-ubiquitin interaction, overview. Molecular dynamics simulations for E2-25K and the Q126L/D127G active site gate mutants Homo sapiens