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Literature summary for 2.3.2.23 extracted from

  • Lei, L.; Bandola-Simon, J.; Roche, P.A.
    Ubiquitin-conjugating enzyme E2 D1 (Ube2D1) mediates lysine-independent ubiquitination of the E3 ubiquitin ligase March-I (2018), J. Biol. Chem., 293, 3904-3912 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information Ube2D1 enzyme knockdown by specific shRNA. Treatment of HeLa cells with Ube2D1 shRNA results in a over 90% reduction in Ube2D1 protein and a 3fold increase in March-I expression, as determined by quantitative immunoblot analysis. The increase in March-I protein expression in Ube2D1 knockdown cells is correlated precisely with reduced ubiquitination of March-I Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine Homo sapiens
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[E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
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?

Organism

Organism UniProt Comment Textmining
Homo sapiens P51668
-
-

Source Tissue

Source Tissue Comment Organism Textmining
B-lymphocyte
-
Homo sapiens
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HeLa cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine
-
Homo sapiens [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
-
?

Synonyms

Synonyms Comment Organism
UBE2D1
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Homo sapiens
ubiquitin-conjugating enzyme E2 D1
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Homo sapiens

General Information

General Information Comment Organism
malfunction knocking down ubiquitin-conjugating enzyme E2 D1 (Ube2D1) impairs March-I ubiquitination, increased March-I expression, and enhanced March-I-dependent down-regulation of MHC-II proteins Homo sapiens
metabolism March-I is ubiquitinated and degraded in the endocytic pathway involving enzyme UBE2D2 Homo sapiens
physiological function ubiquitin-conjugating enzyme E2 D1 (Ube2D1) mediates lysine-independent ubiquitination of the E3 ubiquitin ligase March-I, which contributes to March-I turnover. March-I is a membrane-bound E3 ubiquitin ligase belonging to the membrane-associated RING-CH (March) family. March-I ubiquitinates and downregulates the expression of major histocompatibility complex (MHC) class II and cluster of differentiation 86 (CD86) in antigen-presenting cells. Molecular mechanism regulating March-I ubiquitination, overview. March-I is not ubiquinated on a lysine residue. March-I E3 ligase activity is not required for its ubiquitination and does not regulate March-I protein expression. March-I does not undergo autoubiquitination. Ube2D1 together with another E3 ubiquitin ligase regulates March-I expression. March-I is oligo-ubiquitinated and undergoes proteolytic degradation Homo sapiens