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Literature summary for 2.3.2.23 extracted from

  • Mizushima, N.
    The ubiquitin E2 enzyme UBE2QL1 mediates lysophagy (2019), EMBO Rep., 20, e49104 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.23

Protein Variants

Protein Variants Comment Organism
additional information generation of UBE2QL1-knockdown cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
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lysosome damaged Homo sapiens 5764
-
additional information enzyme UBE2QL1 is diffusely present in the cytosol in untreated cells and translocates to damaged lysosomes Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine Homo sapiens K48- and K63-linked ubiquitination [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens A1L167
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine K48- and K63-linked ubiquitination Homo sapiens [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 ?
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine ubiquitin K48- and K63-linked ubiquitination Homo sapiens [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 ?

Synonyms

Synonyms Comment Organism
E2 enzyme
-
 Homo sapiens
UBE2QL1
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 Homo sapiens
ubiquitin-conjugating enzyme E2
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction without this enzyme, the clearance of ruptured lysosomes is compromised not only upon lysosomal damage but also under normal conditions, revealing its adaptive and constitutive functions. Depletion of the E2 enzyme UBE2QL1 successfully inhibits damage-induced lysosomal ubiquitination. UBE2QL1 depletion affection is greater for K48-linked ubiquitination (K48-Ub) than K63-linked ubiquitination (K63-Ub) Homo sapiens
metabolism the enzyme is involved in lysophagy induced by lysosomal membrane rupture, pathway overview Homo sapiens
physiological function although damaged lysosomes with ruptured membranes can be repaired, these dangerous organelles are also selectively eliminated by autophagic degradation termed lysophagy. This process is initiated by ubiquitination of lysosomal proteins. The E2 enzyme UBE2QL1 catalyzes ubiquitination of damaged lysosomes. UBE2QL1-mediated ubiquitination of lysosomal proteins is crucial for lysophagy following various types of lysosomal damage. L-leucyl-L-leucine methyl ester (LLOMe) treatment induces both ubiquitin K48- and K63-linked ubiquitination through damage of lysosomal membranes. UBE2QL1 has a constitutive housekeeping role in lysosomal homeostasis. UBE2QL1-dependent ubiquitination recruits VCP and SQSTM1 and induces autophagosome formation. Identification of lysosomal membrane proteins, including LIMP2, NPC1, LAMP1, and LAMP2, as potential targets of ubiquitination. UBE2QL1 itself might recognize membrane pores of damaged lysosomes or it might be recruited by an E3 enzyme Homo sapiens