KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000073 | - |
[ubiquitin-carrier-protein Ubc2b]-L-cysteine | mutant D576N, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.000089 | - |
[ubiquitin-carrier-protein Ubc2b]-L-cysteine | mutant K528A, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.000111 | - |
[ubiquitin-carrier-protein Ubc2b]-L-cysteine | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.000122 | - |
[ubiquitin-carrier-protein Ubc2b]-L-cysteine | mutant D576E, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.000135 | - |
[ubiquitin-carrier-protein Ubc2b]-L-cysteine | mutant D576A, pH 7.5, temperature not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | kinetics for Uba1a-catalyzed transthiolation of Ubc2b are used as a reporter assay for determining the Km and kcat values for the three cosubstrates of the ubiquitin-activating enzyme. The E2 transthiolation assays are more sensitive to the potential presence of trace catalytically active fragments than the single turnover end point assays used for quantitating ternary complex stoichiometry | Homo sapiens | ? | - |
? | |
[ubiquitin-activating protein Uba1a]-S-ubiquitinyl-L-cysteine + [ubiquitin-carrier-protein Ubc2b]-L-cysteine | - |
Homo sapiens | [ubiquitin-activating protein Uba1a]-L-cysteine + [ubiquitin-carrier-protein Ubc2b]-S-ubiquitinyl-L-cysteine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | 1 | [ubiquitin-carrier-protein Ubc2b]-L-cysteine | mutant K528A, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.022 | - |
[ubiquitin-carrier-protein Ubc2b]-L-cysteine | mutant D576N, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.1 | - |
[ubiquitin-carrier-protein Ubc2b]-L-cysteine | mutant D576E, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.12 | - |
[ubiquitin-carrier-protein Ubc2b]-L-cysteine | mutant D576A, pH 7.5, temperature not specified in the publication | Homo sapiens | |
3.4 | - |
[ubiquitin-carrier-protein Ubc2b]-L-cysteine | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens |