Literature summary for 2.3.2.15 extracted from

Uraguchi, S.; Tanaka, N.; Hofmann, C.; Abiko, K.; Ohkama-Ohtsu, N.; Weber, M.; Kamiya, T.; Sone, Y.; Nakamura, R.; Takanezawa, Y.; Kiyono, M.; Fujiwara, T.; Clemens, S.
Phytochelatin synthase has contrasting effects on cadmium and arsenic accumulation in rice grains (2017), Plant Cell Physiol., 58, 1730-1742 .

Search for more literature for 2.3.2.15

Cloned(Commentary)

Cloned (Comment)	Organism
gene PCS1, isolation of four different transcript variants of OsPCS1, and amino acid sequence determination and analysis, quantitative real-time RT-PCR enzyme expression analysis revealing that expression of the longest OsPCS1 variant, OsPCS1full, is most abundant, functional recombinant in expression in PCS-deficient	Oryza sativa Japonica Group
gene PCS1, isolation of four different transcript variants of OsPCS1, and amino acid sequence determination and analysis, quantitative real-time RT-PCR enzyme expression analysis revealing that expression of the longest OsPCS1 variant, OsPCS1full, is most abundant, functional recombinant in expression in PCS-deficient mutants of Schizosaccharomyces pombe PCS knockout strain DELTApcs and Arabidopsis thaliana null mutant cad1-3 plants with OsPCS1full possessing good PCS activity in response to As(III) and Cd while the activity of the other PCS variants is very low	Oryza sativa Japonica Group
gene PCS1, isolation of four different transcript variants of OsPCS1, and amino acid sequence determination and analysis, quantitative real-time RT-PCR enzyme expression analysis revealing that expression of the longest OsPCS1 variant, OsPCS1full, is most abundant, functional recombinant in expression in PCS-deficient mutants of Schizosaccharomyces pombe PCS knockout strain DELTApcs and Arabidopsis thaliana null mutant cad1-3 plants with OsPCS1full possessing good PCS activity in response to As(III) and Cd while the activity of the other PCS variants is very low. Transcript of the introduced OsPCS genes is evident in all cad1-3/OsPCS lines except for cad1-3/OsPCS1b line	Oryza sativa Japonica Group
gene PCS1, isolation of four different transcript variants of OsPCS1, DNA and amino acid sequence determination and analysis, quantitative real-time RT-PCR enzyme expression analysis revealing that expression of the longest OsPCS1 variant, OsPCS1full, is most abundant, functional recombinant in expression in PCS-deficient mutants of Schizosaccharomyces pombe PCS knockout strain DELTApcs and Arabidopsis thaliana null mutant cad1-3 plants with OsPCS1full possessing good PCS activity in response to As(III) and Cd while the activity of the other PCS variants is very low. Phenotypes, overview	Oryza sativa Japonica Group
gene PCS2, isolation of a single transcript form of OsPCS2, and amino acid sequence determination and analysis, quantitative real-time RT-PCR enzyme expression analysis, functional recombinant in expression in PCS-deficient mutants of Schizosaccharomyces pombe PCS knockout strain DELTApcs and Arabidopsis thaliana null mutant cad1-3 plants, the activity of PCS2 in response to As(III) and Cd is very low	Oryza sativa Japonica Group

Protein Variants

Protein Variants	Comment	Organism
additional information	identification of two independent OsPCS1 mutant rice lines, a T-DNA and a Tos17 insertion line. The mutant rice plants grown in soil with environmentally relevant As and Cd concentrations show increased As accumulation and decreased Cd accumulation in grains of the T-DNA line. The Tos17 mutant also exhibits the reduced Cd accumulation phenotype. These contrasting effects on As and Cd distribution to grains suggest the existence of at least partially distinct PC-dependent pathways for As and Cd	Oryza sativa Japonica Group
additional information	identification of two independent OsPCS1 mutant rice lines, a T-DNA and a Tos17 insertion line. Thee mutant rice plants grown in soil with environmentally relevant As and Cd concentrations show increased As accumulation and decreased Cd accumulation in grains of the T-DNA line. The Tos17 mutant also exhibits the reduced Cd accumulation phenotype. These contrasting effects on As and Cd distribution to grains suggest the existence of at least partially distinct PC-dependent pathways for As and Cd	Oryza sativa Japonica Group

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa Japonica Group	A0A1Q2SVY7	variant OsPCS1full	-
Oryza sativa Japonica Group	A0A224ASC9	variant OsPCS1c	-
Oryza sativa Japonica Group	B7EJX7	variant OsPCS1a	-
Oryza sativa Japonica Group	Q0DI55	-	-
Oryza sativa Japonica Group	Q5VRI1	variant OsPCS1b	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
seed	-	Oryza sativa Japonica Group	-
seedling	-	Oryza sativa Japonica Group	-

Subunits

Subunits	Comment	Organism
More	the OsPCS1a variant consists of only the N-terminal domain	Oryza sativa Japonica Group
More	the OsPCS1b variant consists of only the C-terminal domain	Oryza sativa Japonica Group
More	the OsPCS1c variant consists only of a very short fragment of the catalytic N-terminal domain	Oryza sativa Japonica Group
More	the OsPCS1full variant shows an AtPCS1/2-like structure with two domains	Oryza sativa Japonica Group
More	the OsPCS2 structure lacks the C-terminal domain	Oryza sativa Japonica Group

Synonyms

Synonyms	Comment	Organism
OsPCS1a	-	Oryza sativa Japonica Group
OsPCS1b	-	Oryza sativa Japonica Group
OsPCS1c	-	Oryza sativa Japonica Group
OsPCS1full	-	Oryza sativa Japonica Group
OsPCS2	-	Oryza sativa Japonica Group
PCS	-	Oryza sativa Japonica Group
phytochelatin synthase	-	Oryza sativa Japonica Group
phytochelatin synthase 1	-	Oryza sativa Japonica Group
phytochelatin synthase 2	-	Oryza sativa Japonica Group

General Information

General Information	Comment	Organism
malfunction	the As(III)-sensitive phenotype of cad1-3 seedlings is completely rescued by the introduction of OsPCS1full variant, whereas growth of the plants expressing the other OsPCS variants is inhibited by As(III) as strongly as that of cad1-3. Similarly, OsPCS1full introduction complements the Cd-sensitive phenotype of cad1-3, while the other OsPCS1 variants do not. OsPCS1 mutant plants show increased sensitivity to Cd and As(III) stress	Oryza sativa Japonica Group
malfunction	the As(III)-sensitive phenotype of cad1-3 seedlings is completely rescued by the introduction of OsPCS1full variant, whereas growth of the plants expressing the other OsPCS1 variants is inhibited by As(III) as strongly as that of cad1-3. Similarly, OsPCS1full introduction complements the Cd-sensitive phenotype of cad1-3, while the other OsPCS1 variants do not	Oryza sativa Japonica Group
malfunction	the As(III)-sensitive phenotype of cad1-3 seedlings is completely rescued by the introduction of OsPCS1full variant, whereas growth of the plants expressing the other OsPCS1 variants is inhibited by As(III) as strongly as that of cad1-3. Similarly, OsPCS1full introduction complements the Cd-sensitive phenotype of cad1-3, while the other OsPCS1 variants do not. OsPCS1 mutant plants show increased sensitivity to Cd and As(III) stress	Oryza sativa Japonica Group
malfunction	the As(III)-sensitive phenotype of cad1-3 seedlings is completely rescued by the introduction of OsPCS1full variant, whereas growth of the plants expressing the other OsPCS1 variants is inhibited by As(III) as strongly as that of cad1-3. Similarly, OsPCS1full introduction complements the Cd-sensitive phenotype of cad1-3, while the other OsPCS1 variants do not. OsPCS1 mutant plants show increased sensitivity to Cd and As(III) stress. Distribution of As and Cd is altered in shoot and grains of OsPCS1 mutants	Oryza sativa Japonica Group
metabolism	contrasting effects of enzyme mutation on As and Cd distribution to grains suggest the existence of at least partially distinct PC-dependent pathways for As and Cd	Oryza sativa Japonica Group
physiological function	phytochelatin synthase has contrasting effects on cadmium and arsenic accumulation in rice grains	Oryza sativa Japonica Group
physiological function	phytochelatin synthase has contrasting effects on cadmium and arsenic accumulation in rice grains. Physiological role of Oryza sativa phytochelatin synthase 1 (OsPCS1) in the distribution and detoxification of arsenic (As) and cadmium (Cd), importance of OsPCS1-dependent PC synthesis for rice As(III) and Cd tolerance	Oryza sativa Japonica Group
physiological function	phytochelatin synthase has contrasting effects on cadmium and arsenic accumulation in rice grains. Physiological role of Oryza sativa phytochelatin synthase 1full (OsPCS1full) in the distribution and detoxification of arsenic (As) and cadmium (Cd), importance of OsPCS1-dependent PC synthesis for rice As(III) and Cd tolerance	Oryza sativa Japonica Group

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Release 2023.1 (January 2023)